左归饮与肾阴虚证方证相应机理的蛋白质组学研究  

Proteome Studies on Mechanisms of Zuoguiyin and Kidney-Yin Deficiency on FANG-ZHENG correspondence

作　　者：李俊丽[1] 李涢[1] 刘铭福[1] 张杰[1] 

机构地区：[1]中国中医科学院中医基础理论研究所,北京100700

出　　处：《中国中医基础医学杂志》2010年第3期203-205,208,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：北京市自然科学基金资助项目(7082068)

摘　　要：目的:采用蛋白质组学技术在蛋白质表达水平探讨左归饮与肾阴虚证方证相应的机理。方法:将SD大鼠随机分为正常对照组、肾阴虚证模型组与左归饮组3组,对其肝、肾组织蛋白质提取液及血清分别进行蛋白质双向电泳并对电泳图谱进行分析,找出左归饮调节纠正的蛋白质,并进行质谱分析鉴定。结果:找出左归饮调节纠正的蛋白质点21个,质谱鉴定出18种蛋白质。结论:左归饮可以调节纠正肾阴虚证模型动物有关的糖代谢、脂肪代谢、信号传导等酶类非正常表达的蛋白质。Objective： To explore the mechanisms of Zuoguiyin to cure kidney-yin deficiency using proteomic technique on the expression levels of protein.Methods： SD rats were randomly divided into three groups as follows： normal group、model group and treatment group.The proteins of liver tissue、 kidney tissue and serum were separated and analysed by two-dimensional gel（2-DE） to find regulated proteins by Zuoguiyin.The mass spectrometry was used to identify these proteins.Results： 21 differentially expressed protein spots were detected,and 18 proteins spots were identify.Conclusion： Zuoguiyin could regulate differentially expressed protein of model rats with kidney-yin deficiency,which include glucose metabolism,lipid metabolism,signal transduction,antioxidation.

关 键 词：左归饮 肾阴虚证 方证相应 蛋白质组学 

分 类 号：R285.5[医药卫生—中药学]