γ射线照射对人肌腱组织形态学及羟脯氨酸含量的影响(英文)  

Effect of γ-rays on the Histomorphology and Hydroxyproline Content of Human Tendon

作　　者：王华磊[1] 沈卫[2] 张积华[3] 王沛涛[4] 田少奇[1] 夏长所[1] 张才龙[1] 孙康[1] 

机构地区：[1]青岛大学医学院附属医院关节外科,山东青岛266003 [2]青岛大学医学院附属医院中西医结合教研中心,山东青岛266003 [3]青岛大学医学院附属医院查体中心,山东青岛266003 [4]青岛大学医学院附属医院深低温中心,山东青岛266003

出　　处：《现代生物医学进展》2010年第1期57-61,F0003,共6页Progress in Modern Biomedicine

基　　金：Natural Science Foundation of Shandong Province(No.Z2008C10)~~

摘　　要：目的:研究2.87Mrad剂量γ射线照射后,肌腱组织形态学及羟脯氨酸含量的变化。方法:选取40根人新鲜上肢掌深屈肌腱,-80℃深低温冷冻6周,等分为二:实验组(A组),对照组(B组)。A组肌腱在干冰低温保存条件下行γ射线照射,检测肌腱最终吸收剂量为2.87Mrad。分别行HE、VG染色,在普通光镜和透射电镜下观察其组织形态学变化。用柱前衍生高效液相色谱检测肌腱胶原蛋白水解液中羟脯氨酸含量。结果:①形态学观察发现,同非照射组肌腱相比,照射组肌腱胶原纤维束间隙较大,纤维排列卷曲紊乱,并可见部分断裂。电镜下胶原纤维横纹模糊消失,腱细胞膜溶解消失,核崩解,细胞器减少。②在同等水解条件下,胶原蛋白水解液中照射组羟脯氨酸含量与非照射组相比,有显著差异(P<0.05)。结论:2.87Mrad剂量γ射线照射可引起肌腱组织形态结构发生显著改变。在同等水解条件处理下,照射组肌腱胶原蛋白水解液中羟脯氨酸含量显著高于非照射组。Objective：To investigate the influence of 2.87 Mrad γ-rays on the histomorphology and content of hydroxyproline of human tendon.Methods：Forty fresh human tendons were freezed in -80℃ for six weeks,and randomly divided into two groups：the experimental group（group A） and control group（group B）.Group A stored in dry ice was exposed to γ-rays until the absorbed dose reached 2.87 Mrad.The tendons was stained with HE and VG methods.And then they were detected by common light microscope and transmission electron microscope respectively.The hydroproline in tendon collagen hydrolysate was determined by the high performance liquid chromatography with precolumn derivation.Results：① Compared to the control group,the gaps between collagen fiber bands in irradiation group were enlarged,incompact,disordered and some of them fractured.The transverse striation of the collagen fibers became unclear or disappeared.The membrane of tendon cell dissolved,the cell nuclear disintegrated and the organelles reduced.② The hydroxyproline in the collagen hydrolysate of irradiation group was significantly higher than that in non-irradiation group under the same hydrolysis condition（P0.05）.Conclusion：The morphology and structure of tendon changed significantly after 2.87 Mrad dose of γ-rays irradiation.The hydroxyproline in collagen hydrolysate of irradiation group is significantly greater than that of non-irradiation group under the same hydrolysis condition.

关 键 词：Γ射线 人肌腱 组织形态 羟脯氨酸 高效液相色谱 

分 类 号：R318.08[医药卫生—生物医学工程]