α-平滑肌肌动蛋白在人胸主动脉夹层中的表达  

Expression of α-smooth muscle actin in human's thoracic aorta dissection

作　　者：侯乐伟[1] 廖明芳[1] 翁剑锋[1] 杨琳[1] 邹思力[1] 景在平[1] 

机构地区：[1]第二军医大学长海医院血管外科,全军血管外科研究所,上海200433

出　　处：《现代生物医学进展》2010年第1期113-114,124,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(3060599)

摘　　要：目的:探讨胸主动脉壁中α-平滑肌肌动蛋白(α-SMA)的表达与胸主动脉夹层(TAD)的关系。方法:采集人TAD的动脉壁组织和正常人胸主动脉壁组织,采用Western Blotting和免疫组织化学方法检测α-SMA在组织中的表达程度。结果:在DA组中,α-SMA的表达明显减少,血管平滑肌细胞(VSMC)以增殖表型为主。结论α-SMA的减少主要发生在血管中膜层的VSMC中,细胞发生表型变化,导致中膜弹性变差,发生夹层病变。因此,α-SMA可能在TAD的发病中具有重要作用,值得进一步深入探讨。Objective：To explore the relationship of α-smooth muscle actin（α-SMA） in human＇s thoracic aorta dissection（TAD）.Methods：Tissue samples of dissected thoracic aorta（DA） and normal thoracic aorta（NA） were evaluated.Western Blotting and immunohistochemical studies were performed to detected α-SMA expression in tissues.Results：α-SMA expression was apparently downregulated in DA group,while proliferated phenotype was predominated among vascular smooth muscle cells.Conclusion：Expression of α-SMA was mainly downregulated in VSMC of vascular media wall.Phenotype change of VSMC was occurred sent so that elasticity of the media was lack and dissection of vascular was performed.Therefore,α-SMA might play a key role in the development of human＇s thoracic aorta dissection,which is need to be further explored.

关 键 词：Α-平滑肌肌动蛋白 人胸主动脉夹层 血管平滑肌细胞 

分 类 号：Q291[生物学—细胞生物学] R543.1[医药卫生—心血管疾病]