包裹于PLGA微球中用于蛋白控释的多糖纳米颗粒  

Microencapsulated Protein-loaded Dextran Nanoparticles in PLGA Microspheres for Protein-controlled Release

作　　者：赵昊[1] 任甜甜[1] 陈唯娟[1] 洪晓芸[1] 袁伟恩[1] 

机构地区：[1]上海交通大学,上海200240

出　　处：《现代生物医学进展》2010年第1期130-132,共3页Progress in Modern Biomedicine

基　　金：国家自然基金(30873180);上海市科研课题基金(0952nm03700)

摘　　要：目的:研究包裹在PLGA微球中的多糖纳米颗粒在保护蛋白稳定性和改善药物体外释放行为方面的作用。方法:将模型药物BSA用低温诱导相分离方法担载于多糖纳米颗粒之中,后将其用水包油包固复乳法包裹于PLGA微球内。应用体积排阻色谱(SEC-HPLC)和红外色谱(FTIR)表征蛋白的稳定性,而且也研究了样品的体外释放行为。结果:这种方法能够很好的保护蛋白的稳定性,保持蛋白结构在制备过程中不会改变,而且改善了体外释放行为,减少了突释。结论:多糖纳米颗粒结合PLGA微球能够提供一种有效的解决蛋白控释的途径。Objective：To investigate the effect of microencapsulation of protein drugs-loaded dextran nanoparticles into poly（D,L-lactic-co -glycolic acid）（PLGA） microspheres on integrity of protein drugs,and improvement of release profiles of protein drugs in vitro.Methods：Bovine serum albumin（BSA）-loaded dextran nanoparticles were prepared by the method of freezing-induced phase separation,then the protein-loaded dextran nanoparticles were microencapsulated into PLGA microspheres by solid-in-oil-in-water（S/O/W） multiple emulsion technique.The samples were characterized by size exclusion HPLC（SEC-HPLC） and Fourier-transform infrared spectroscopy（FTIR）.In vitro release profiles of the samples were also detected.Results：The integrity of BSA was protected perfectly by this method and the release behavior of the microspheres were improved.Conclusions：This method is a effective technique for protein-controlled release.

关 键 词：葡聚糖 BSA PLGA微球 控释 多糖纳米颗粒 

分 类 号：O629[理学—有机化学] R944.2[理学—化学]