人外周血T淋巴细胞凋亡与胀亡研究及氨茶碱的诱导作用  

The Study of Apoptosis and Oncosis in human peripheral blood T lymphocyte and aminophylline’s induction

作　　者：申严[1] 黄瑾[2] 郑晓滨[2] 叶于阗[2] 

机构地区：[1]深圳市龙岗中心医院,广东深圳518116 [2]中山大学附属第五医院,广东珠海519000

出　　处：《现代生物医学进展》2010年第3期462-465,共4页Progress in Modern Biomedicine

摘　　要：目的:在前期研究的基础上进一步观察氨茶碱对人外周血T淋巴细胞凋亡与胀亡的诱导作用。方法:密度梯度离心法及尼龙棉柱法分离健康成年人外周血T淋巴细胞,分空白组及氨茶碱组,培养后观察细胞光镜及电镜形态学、FDA/PI荧光染色,并用流式细胞仪检测凋亡和胀亡细胞比例变化。结果:①人外周血T淋巴细胞经体外培养,可自然出现典型的细胞凋亡与胀亡形态学改变。②人外周血T淋巴细胞在氨茶碱诱导作用下,凋亡率有显著增高,但胀亡率相对无显著增高。结论:人外周血T淋巴细胞存在凋亡与胀亡现象,氨茶碱可诱导人外周血T淋巴细胞凋亡,但不能有效的诱导健康人外周血T淋巴细胞胀亡。Objective：To observe apoptosis and oncosis in human peripheral blood T lymphocyte after preliminary study. Methods ： Peripheral blood T lymphocyte of healthy adult was separated with Percoll （1.073g/mL） and harvested by using nylon column. The cultured cells were divided into control and aminophylline group, and cell morphology was observed through light microscope, electron microscope and fluorescent microscope. Incidence rate of apoptosis and oncosis was analyzed by flow cytometry. Results： ①Typical apoptosis and oncosis morphology could be observed in T lymphocyte T subgroups of healthy control peripheral blood in vitro culture. ② Induced by aminophylline, the apoptosis of T lymphocyte had been increased, but the oncosis rate hadn’t enhanced significantly. Conclusions：Apoptosis and oncosis can be seen in human peripheral blood T lymphocytes, aminophylline could induce apoptosis, but couldn’t enhance oncosis rate significantly.

关 键 词：凋亡 胀亡 T淋巴细胞 氨茶碱 

分 类 号：Q813[生物学—生物工程]