枸杞对慢性心理应激大鼠海马CA3区锥体细胞的影响  

Effect of Lycium Chinensis on Hippocampal Neurons of CA3 Region Induced by Chronic Psychological Stress in Rats

作　　者：周健[1] 杨惠芳[1] 朱玲勤[1] 李宏辉[1] 高晓燕[1] 张鹏举[1] 

机构地区：[1]宁夏医科大学公共卫生学院,宁夏银川750004

出　　处：《工业卫生与职业病》2010年第2期83-86,共4页Industrial Health and Occupational Diseases

基　　金：教育部科学技术研究重点项目(206162);宁夏自然科学基金(NZ0797)

摘　　要：目的探讨枸杞对慢性心理应激大鼠海马CA3区锥体细胞数量和形态的影响。方法选择健康成年SD大鼠96只,雌雄各半,随机分为8组。采用慢性复合应激的方法,通过HE染色方法观察枸杞对实验大鼠海马锥体细胞的影响。结果应激组、枸杞低剂量+应激组和枸杞高剂量+应激组血清皮质醇含量明显升高,与对照组比较,差异有统计学意义(P<0.01)。枸杞低剂量组、枸杞中剂量组、枸杞高剂量组、枸杞中剂量+应激组和枸杞高剂量+应激组血清皮质醇含量明显降低,与应激组比较,差异有统计学意义(P<0.01)。应激组CA3区细胞数明显减少,与对照组比较,差异有统计学意义(P<0.01)。枸杞低剂量+应激组、枸杞中剂量+应激组和枸杞高剂量+应激组CA3区细胞数明显增多,与应激组比较,差异有统计学意义(P<0.05)。光镜下观察应激组细胞排列不整齐、间隙增宽,部分胞核固缩呈三角形或不规则形;枸杞低剂量组、枸杞中剂量组、枸杞高剂量组海马细胞形态与对照组基本相同,枸杞低剂量+应激组、枸杞中剂量+应激组、枸杞高剂量+应激组海马细胞形态介于对照组和应激组之间。结论慢性心理应激引起血清皮质醇含量升高,导致大鼠海马CA3区锥体细胞受损;枸杞能够降低应激大鼠的血清皮质醇水平,减缓应激损伤海马,对受损伤的神经元具有一定的保护作用。Objective To study the effect of Lycium chinensis on the quantity and morphology of hippocampal neurons of CA3 region in rats induced by chronic psychological stress.Methods96 SD rats were divided into eight groups.Chronic compound psychological stress was adopted.The morphological changes of hippocampal neurons of CA3 region were observed by HE staining.ResultsThe serum cortisol levels of stress group,low dose Lycium chinensis ＋ stress group and high dose Lycium chinensis＋stress group were significantly higher than those of the control group（P〈0.01）.The serum cortisol levels of low,medium,and high doses Lycium chinensis groups,medium dose Lycium chinensis＋stress and high dose Lycium chinensis ＋ stress group were significantly lower than those of the stress group（P〈0.01）.Comparing with the control group,the quantity of hippocampal of neurons CA3 region of the stress group significantly decreased（P〈0.01）.Comparing with the stress group,the quantity of hippocampal neurons of CA3 region of low,medium and high doses Lycium chinensis＋stress groups increased significantly（P〈0.05）.Cells in stress group irregularly lined,widened gap,some of nuclei condensed into triangular or irregular shapes were observed under light microscope.The morphology of low,medium,and high doses groups was basically the same with the control group.In low,medium and high doses＋stress groups,the hippocampal cells patterns were in-between the control group and the stress group.ConclusionsChronic psychological stress may induce the serum cortisol levels to rise causing the damage of hippocampal CA3 pyramidal cell.Lycium chinensis may reduce the levels of serum cortisol.By reducing the injury of stressed hippocampus,there would be protective effect on injured nerve element.

关 键 词：枸杞 慢性心理应激 海马 CA3区 锥体细胞 

分 类 号：R13[医药卫生—劳动卫生]