马钱子碱中毒脑神经细胞Bcl-2和Caspase-3表达的研究  

Study on Bcl-2 and Caspase-3 Expression in Brain Nervous Cell after Brucine Poisoning

作　　者：雷怀成[1] 姚新[2] 刘涛[3] 

机构地区：[1]郧阳医学院,湖北十堰442000 [2]十堰市公安局,湖北十堰442000 [3]郧阳医学院附属太和医院,湖北十堰442000

出　　处：《工业卫生与职业病》2010年第2期90-93,共4页Industrial Health and Occupational Diseases

摘　　要：目的观察马钱子碱中毒后不同时间、不同剂量Bcl-2和Caspase-3表达的变化。方法选用20只Wistar大鼠为正常对照组,44只Wistar大鼠复制马钱子中毒模型,应用免疫组织化学和图像分析方法研究大鼠脑神经细胞马钱子中毒后Bcl-2与Caspase-3蛋白表达的变化规律。结果大鼠在马钱子中毒低、中剂量后的1-7 d不同时间段内,脑神经细胞Bcl-2与Caspase-3蛋白阳性表达细胞数均高于正常对照组,与相应的对照组比较,差异均有统计学意义（P〈0.05）。结论Bcl-2与Caspase-3参与了马钱子中毒的病理生理过程。Objective To observe changes in expression of Bcl-2 and Caspase-3 under different times and dosages after brucine poisoning.Methods20 Wistar rats were taken as the normal control group and 44 Wistar rats as brucine poisoning model group.Changes in expression of Bcl-2 and Caspase-3 in the rat brain cells after brucine poisoning were investigated using immunohistochemistry and computer competitive image analysis system.ResultsIn the different time periods of 1-7 days after low and medium dosages brucine poisoning,the cell numbers of positive expression of Bcl-2 and Caspase-3 were higher than those of the normal control.The differences were statistically significant（P〈0.05）.ConclusionsBcl-2 and Caspase-3 were involved in the pathophysiological process of rat brucine poisoning.

关 键 词：马钱子 中毒 BCL-2 CASPASE-3 

分 类 号：R114[医药卫生—卫生毒理学]