鳗弧菌(Vibrio anguillarum)侵染对青蛤(Cyclina sinensis)磷酸酶活性的影响  

EFFECT OF VIBRIO ANGUILLARUM ON ACTIVITY OF PHOSPHATASE IN CYCLINA SINENSIS

作　　者：宋欣[1] 张丽岩[1] 高玮玮[1] 潘宝平[1] 

机构地区：[1]天津师范大学化学与生命科学学院细胞遗传与分子调控天津市重点实验室

出　　处：《海洋与湖沼》2010年第2期254-258,共5页Oceanologia Et Limnologia Sinica

基　　金：天津市科委应用基础与前沿技术重点项目资助;09JCZDJC19300号;天津市科委应用基础研究面上项目资助;06YFJMJC11800号

摘　　要：对500个成体青蛤分别用鳗弧菌和生理盐水注射,在感染后3h、6h、12h、24h和36h分别取不同处理组的肝胰脏、鳃和闭壳肌组织,测定上述样品的碱性磷酸酶(ALP)及酸性磷酸酶(ACP)活性,分析鳗弧菌对青蛤体内免疫相关酶活性的影响。结果表明,鳗弧菌感染组的ALP与ACP活性从高到低依次为肝胰脏>鳃>闭壳肌,其中青蛤肝胰脏和鳃组织中ALP和ACP活性均有显著升高的趋势,并且在12h时达到最高,与对照组差异显著(P<0.05),随后呈现下降趋势。而青蛤闭壳肌组织中侵染组ALP和ACP活性与对照组之间没有显著差异(P>0.05)。鳗弧菌对青蛤的磷酸酶活性影响较大,对其免疫防御系统有明显的刺激作用。In this study, Vibrio anguillarum and normal saline were injected separately into 500 adult clams （Cyclina sinensis）. At 3h, 6h, 12h, 24h, and 36h after injection, hepatopancreas, gills, and adductor muscle tissues of the samples in different treatment groups were collected, and alkaline phosphatase （ALP） and acid phosphatase （ACP） activities were measured in order to reveal the relationship between V. anguillarum injection and immune-related response. The results show that after the injection, ALP and ACP activities in the clam tissue were, in a descending order, hepatopancreas 〉 gill 〉 adductor muscle tissue. After the injection, the activities in hepatopancreas and gill tissues increased significantly, and reached its highest level at 12h and then decreased. In the adductor muscle tissues, the activities showed no significant difference compared with the control group. Our research shows that V. anguillarum has noticeable impact on phosphatase of C. sinensis and significant stimulating effect to the clam＇s immune system.

关 键 词：青蛤 鳗弧菌 碱性磷酸酶(ALP) 酸性磷酸酶(ACP) 

分 类 号：Q789[生物学—分子生物学]