SPA-ELISA 一步法检测囊虫病患者血清特异性抗体的研究  

Study on detecting specific antibody in cysticercosis patients by one step SPA ELISA.

作　　者：陈盛霞[1] 周慧娟[1] 帅连云[1] 徐会娟[1] 张永红[2] 李浩[2] 裘丽姝[2] 姜本启 邵士才 杨新成 

机构地区：[1]镇江医学院 [2]中国预防医学科学院寄生虫病研究所 [3]徐州市防疫站

出　　处：《临床检验杂志》1998年第6期330-332,共3页Chinese Journal of Clinical Laboratory Science

基　　金：江苏省卫生厅医学科技发展基金

摘　　要：用ＳＰＡ－ＥＬＩＳＡ一步法检测了囊虫病患者血清特异性抗体，并与快速ＳＰＡ－ＥＬＩＳＡ进行对比。两法同时检测了５９例囊虫病患者，３６例非囊虫病病人和３０例正常人血清。结果表明：ＳＰＡ－ＥＬＩＳＡ一步法和快速ＳＰＡ－ＥＬＩＳＡ的阳性率分别为９４．９１％和９８．３１％，几何平均滴度（ＧＭＰ）分别为１∶４２５．８９和１∶５６５．７２，均无显著性差异。对１例肝吸虫病人和１例包虫病人血清均起交叉反应。说明ＳＰＡ－ＥＬＩＳＡ一步法具有与快速ＳＰＡ－ＥＬＩＳＡ相同敏感性和特异性，并且快速、方便、花费少。serum sample of cysticercosis patients were determined by one step SPA ELISA and compared with rapid SPA ELISA. Positive was 94 91%(56/59)and 98 31%(58/59),antibody GMP 1∶425 89 and 1∶565 72 respectively,the difference being statistically insignificant(P>0 05).While 30 serum samples of healthy persons were negative,36 patients other than cysticercosis,only one sample of echinococcusis and one sample of clonorchiasis patients reveled false positive with two methods.The present study has proved that one step SPA ELISA is a sensitive,simple,rapid,savable and useful method for clinical diagnosis and epidemiological investigation on cysticercosis.

关 键 词：囊虫病 血清 抗体 SPA-ELISA 

分 类 号：R532.330.4[医药卫生—内科学] R446.6[医药卫生—临床医学]