油菜田看麦娘对高效氟吡甲禾灵产生抗药性的生理响应  

Physiological responses of Alopecurus aequalis to herbicide haloxyfop-R-methyl

作　　者：黄世霞[1,2] 郑青松[3] 王庆亚[2] 张守栋[2] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036 [2]南京农业大学生命科学学院,南京210095 [3]南京农业大学资源与环境科学学院,南京210095

出　　处：《生态学杂志》2010年第4期674-679,共6页Chinese Journal of Ecology

基　　金：江苏省十五科技攻关项目(BE2001346);安徽农业大学校长基金资助项目(2005)

摘　　要：采用种子检测法,鉴定了对除草剂高效氟吡甲禾灵产生抗药性的看麦娘(Alopecu-rus aequalis Sobol.)生物型JXRII。为探讨看麦娘对高效氟吡甲禾灵产生抗性的生理反应,以敏感生物型JJSII为对比,喷施高效氟吡甲禾灵后,测定抗药生物型和敏感生物型几个重要生理指标变化情况的差异。结果表明:高效氟吡甲禾灵处理下,JJSII的叶片电解质泄漏率、MDA含量极显著上升,叶绿素、类胡萝卜素含量下降;JXRII叶片的电解质渗漏率、MDA含量、叶绿素、类胡萝卜素均与对照无显著差异;高效氟吡甲禾灵处理下,JJSII的GSH含量在处理后就开始逐渐下降,JXRII的GSH含量始终高于对照;二者可溶性糖含量均增加,敏感生物型JJSII可溶性糖含量增加的幅度远远大于抗性生物型JXRII;这2种群体经除草剂胁迫下,除草剂对敏感性生物型的生理变化影响大,抗性生物型表现为一定的生理适应性。Alopecurus aequalis is a troublesome weed in oilseed rape field,and herbicide haloxyfop-R-methyl is often used for its control.A rapid identification by the method of seed bioassay showed that A.aequalis biotype JXRII had the resistance to the herbicide,while biotype type JJSII was in adverse.In order to approach the differences in the physiological responses of the two biotypes to the herbicide,haloxyfop-R-methyl was sprayed on them,and some important physiological indices were determined.Two days after spraying haloxyfop-R-methyl,the leaf glutathione （GSH） content of JJSII decreased gradually,while that of JXRII increased.After spraying haloxyfop-R-methyl for 6 days,the leaf electrolytic leakage and malondialdehyde （MDA） content of JJSII increased markedly,and the chlorophyll （Chl） and carotenoid （Car） contents decreased.On the contrary,the leaf electrolytic leakage and the leaf MDA,Chl,and Car contents of JXRII had no significant differences with the control.Spraying haloxyfop-R-methyl increased the leaf soluble sugar content of the two biotypes,but the increment was much more for JJSII than for JXRII.It was suggested that haloxyfop-R-methyl strongly affected the physiological aspects of susceptible biotype of A.aequalis,while the resistant biotype had some physiological adaptability to the herbicide stress.

关 键 词：高效氟吡甲禾灵 看麦娘 生理指标 抗性生物型 胁迫 

分 类 号：S451.2[农业科学—植物保护]