中国荷斯坦奶牛FASN基因遗传多样性及其与泌乳性能的相关性分析  

Genetic Diversity Analysis of FASN Gene and Its Correlation with Milk Performance in Southern Chinese Holstein

作　　者：武秀香[1] 杨章平[1] 王小龙[1] 施雪奎[1] 毛永江[1] 周里根 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]上海光明荷斯坦牧业有限公司,上海200072

出　　处：《中国畜牧杂志》2010年第1期1-4,共4页Chinese Journal of Animal Science

基　　金：国家高技术发展计划(863)(2008AA10Z144)

摘　　要：哺乳动物脂肪酸合成酶(FASN)基因在脂肪酸合成中起至关重要的作用。研究利用PCR-SSCP方法检测109头中国荷斯坦奶牛FASN基因第2、4、26、34外显子遗传多态性,并对多态性与泌乳性状进行相关性分析。结果表明:仅第34外显子(g.16009)存在A/G多态性,其余3个外显子均未发现多态性;第34外显子有2个等位基因为A(突变点为G)/B(突变点为A),基因频率0.8802/0.1198;最小二乘分析表明,第34外显子不同基因型对乳脂率、体细胞评分(SCS)效应显著(P<0.05)。为进一步探索牛奶中乳脂率调控机制奠定一定基础。Fatty acid synthase （FASN） plays very important role in mammalian fat synthesis. The polymorphism of FASN gene exon 2, exon 4, exon 26, exon 34 in Chinese Holstein population was detected using PCR -SSCP in this study. Results indicated that mutation point was only found in exon 34 （g.16009AG）. Holstein cows in southern China have A （point mutation G） and B （point mutation A） alleles. The frequencies of alleles were 0.8 802/0.1 198, respectively, with genotypic frequencies AA 0.7 604 and AB 0.2 396. GLM model was applied to analyze the impact of FASN gene genotype on milk protein percentage, milk fat percentage, milk yield, and somatic cell score（SCS）. It was also showed that the two genotypes had a significant difference（P 〈0.05） on milk fat, and SCS. This study provided original information for elucidating regulatory mechanism of milk fat.

关 键 词：FASN PCR-SSCP SCS 乳脂率 中国荷斯坦奶牛 

分 类 号：S823.2[农业科学—畜牧学]