中国不同地理来源稻曲病菌rDNA-IGS的初步分析  

Preliminary analysis of rDNA-IGS of Ustilaginoidea virens isolates from different geographical regions in China

作　　者：王永强[1] 樊荣辉[1] 刘兵[1] 李绍慧[1] 郑大伟[1] 张敬泽[1] 胡东维[1] 

机构地区：[1]浙江大学生物技术研究所水稻生物学国家重点实验室,杭州310029

出　　处：《植物病理学报》2010年第2期214-216,共3页Acta Phytopathologica Sinica

基　　金：公益性行业(农业)科研专项(200903039-5);水稻生物学国家重点实验室开放基金资助项目

摘　　要：The rDNA-IGS of 11 Ustilaginoidea virens isolates from different geographical regions in China were amplified by PCR and sequenced. The results showed that the rDNA-IGS fragments consisted of one cen-tral variable region and two lateral conservative regions. The two conservative sequences among the11 isolates shared the sequence similarity of 99.44%. The variable region consisted of 2,4,or 6 of 77 bp-length-repeats in the isolates and the sequences of the repeat units were high conservative. Primers UIGS III and UIGS IV were designed for amplification of the variable region in the rDNA-IGS.The rDNA-IGS of 11 Ustilaginoidea virens isolates from different geographical regions in China were amplified by PCR and sequenced. The results showed that the rDNA-IGS fragments consisted of one central variable region and two lateral conservative regions. The two conservative sequences among the 11 isolates shared the sequence similarity of 99.44 %. The variable region consisted of 2, 4, or 6 of 77 bp-length-repeats in the isolates and the sequences of the repeat units were high conservative. Primers UIGS III and UIGS IV were designed for amplification of the variable region in the rDNA-IGS.

关 键 词：稻曲病菌 RDNA 地理来源 IGS 中国 人工接种技术 发生范围 食用安全 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]