灰枣红点软腐病病原菌的鉴定  

Identification of the pathogen causing fruit soft rot in Ziziphus jujuba Mill.

作　　者：牛晓科[1] 崔建州[1] 侯晓杰[1] 陈谟林[2] 冉隆贤[1] 

机构地区：[1]河北农业大学林学院,保定071001 [2]新郑市枣树科学研究所,新郑451150

出　　处：《植物病理学报》2010年第2期217-221,共5页Acta Phytopathologica Sinica

基　　金：2008年度教育部科学技术研究重点项目(208017);河北农业大学科研基金

摘　　要：The damage and symptoms of jujube fruit soft rot in Xinzheng,Henan Province,were investigated from 2006 to 2008,and the pathogen was identified based on the morphological characteristics and the ITS se-quence of ribosome DNA. The results showed that the aerial mycelium of colony was white in color in the first four days,then turned gray after incubation on PDA for5 -6 d at25℃ and became black two weeks later.The mycelia grew luxuriantly with velvet character. The pycnidium was flask-shaped with a height of 196.9μm and a width of 213.3μm on the avereage. The conidium was colorless with single cell and had the shape of spindle,its size was (15.0 -20.0)μm ×(4.5 -6.5)μm. The conidiophore was fastigiate. The homology of ITS sequence of ribosome DNA between the tested strain NXK and Botryosphaeria dothidea(GenBank ac-cession number:AJ938005) reached 99.87%,with a difference of only two base pairs. Based on the results of both morphological characters and molecular identification,the pathogen of jujube fruit soft rot in Xinzheng was identified as B. dothidea(Moug. ex Fr.) Ces. et de Not..The damage and symptoms of jujube fruit soft rot in Xinzheng, Henan Province, were investigated from 2006 to 2008, and the pathogen was identified based on the morphological characteristics and the ITS sequence of ribosome DNA. The results showed that the aerial mycelium of colony was white in color in the first four days, then turned gray after incubation on PDA for 5 -6 d at 25℃ and became black two weeks later. The mycelia grew luxuriantly with velvet character. The pycnidium was flask-shaped with a height of 196.9 μm and a width of 213.3 μm on the avereage. The conidium was colorless with single cell and had the shape of spindle, its size was （15.0 -20.0）μm × （4.5 -6.5）μ. The conidiophore was fastigiate. The homology of ITS sequence of ribosome DNA between the tested strain NXK and Botryosphaeria dothidea （ GenBank accession number： AJ938005） reached 99.87%, with a difference of only two base pairs. Based on the results of both morphological characters and molecular identification, the pathogen of jujube fruit soft rot in Xinzheng was identified as B. dothidea（Moug, ex Fr. ） Ces. et de Not..

关 键 词：新郑灰枣 软腐病 病原菌 鉴定 河南省 发病率 危害 作者 

分 类 号：S665.1[农业科学—果树学]