Community analysis of ammonia-oxidizing bacteria in activated sludge of eight wastewater treatment systems  

作　　者：Xiaohui Wang Xianghua Wen Craig Criddle George Wells Jie Zhang Yin Zhao 

机构地区：[1]Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China. [2]Department of Civil and Environmental Engineering, Jerry Yang & Akiko Yamazaki Environment & Energy Building, Stanford University, Stanford, CA94305-4020, USA [3]Beijing Drainage Group Co., Ltd., Beijing 100084, China

出　　处：《Journal of Environmental Sciences》2010年第4期627-634,共8页环境科学学报（英文版）

基　　金：supported by the Key Projects in National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period (No.2006BAC19B01-02);the Mega-projects of Science Research for Water (No.2008ZX07313-3);the Program of Introducing Talents of Discipline to Universities

摘　　要：We investigated the communities of ammonia-oxidizing bacteria （AOB） in activated sludge collected from eight wastewater treatment systems using polymerase chain reaction （PCR） followed by terminal restriction fragment length polymorphism （T-RFLP）, cloning, and sequencing of the α-subunit of the ammonia monooxygenase gene （amoA）. The T-RFLP fingerprint analyses showed that different wastewater treatment systems harbored distinct AOB communities. However, there was no remarkable difference among the AOB T- RFLP profiles from different parts of the same system. The T-RFLP fingerprints showed that a full-scale wastewater treatment plant （WWTP） contained a larger number of dominant AOB species than a pilot-scale reactor. The source of influent affected the AOB community, and the WWTPs treating domestic wastewater contained a higher AOB diversity than those receiving mixed domestic and industrial wastewater. However, the AOB community structure was little affected by the treatment process in this study. Phylogenetic analysis of the cloned amoA genes clearly indicated that all the dominant AOB in the systems was closely related to Nitrosomonas spp. not to Nitrosospira spp. Members of the Nitrosomonas oligotropha and Nitrosomonas communis clusters were found in all samples, while members of Nitrosomonas europaea cluster occurred in some systems.We investigated the communities of ammonia-oxidizing bacteria （AOB） in activated sludge collected from eight wastewater treatment systems using polymerase chain reaction （PCR） followed by terminal restriction fragment length polymorphism （T-RFLP）, cloning, and sequencing of the α-subunit of the ammonia monooxygenase gene （amoA）. The T-RFLP fingerprint analyses showed that different wastewater treatment systems harbored distinct AOB communities. However, there was no remarkable difference among the AOB T- RFLP profiles from different parts of the same system. The T-RFLP fingerprints showed that a full-scale wastewater treatment plant （WWTP） contained a larger number of dominant AOB species than a pilot-scale reactor. The source of influent affected the AOB community, and the WWTPs treating domestic wastewater contained a higher AOB diversity than those receiving mixed domestic and industrial wastewater. However, the AOB community structure was little affected by the treatment process in this study. Phylogenetic analysis of the cloned amoA genes clearly indicated that all the dominant AOB in the systems was closely related to Nitrosomonas spp. not to Nitrosospira spp. Members of the Nitrosomonas oligotropha and Nitrosomonas communis clusters were found in all samples, while members of Nitrosomonas europaea cluster occurred in some systems.

关 键 词：activated sludge ammonia-oxidizing bacteria T-RFLP amoA gene wastewater treatment plant 

分 类 号：Q959.468[生物学—动物学] X703[环境科学与工程—环境工程]