共代谢条件下丁基黄药的生物降解实验研究  

Study on co-metabolism biodegradation of butyl xanthate

作　　者：鄢恒珍[1,2] 龚文琪[1] 梅光军[1] 陈绍华[1] 陈晓东[1] 

机构地区：[1]武汉理工大学资源与环境工程学院,湖北武汉430070 [2]湖南城市学院城市建设系,湖南益阳413000

出　　处：《环境污染与防治》2010年第4期1-5,共5页Environmental Pollution & Control

基　　金：国家高技术研究发展计划("863计划")项目(No.2007AA06Z123);国家重点基础研究发展计划("973计划")项目(No.2004CB619204);湖南省科技计划项目(No.2008SK4012);益阳市科技计划项目(No.20070202)

摘　　要：微生物共代谢是降解废水中难降解有机物的重要方式。通过实验研究,比较了以丁基黄药作为唯一碳源和能源以及有共代谢基质存在下丁基黄药的降解过程。结果表明,共代谢显著提高了丁基黄药的降解率。在25℃条件下,以丁基黄药为唯一碳源和能源时,其降解率仅为43.1%;而利用葡萄糖作为共代谢基质,当葡萄糖质量浓度为0.20g/L时,振荡培养72h后丁基黄药的降解率可达65.2%;再加入微量的蛋白胨作为氮源后,丁基黄药的降解率可提高到73.5%;加入共代谢基质后微生物的适应期由原来的36h缩短至24h。共代谢是提高丁基黄药生物降解性能的一条有效途径。The microorganism co-metabolism was an important mode for biodegrading the refractory organics in wastewater. The processes of butyl xanthate biodegradation with and without the existence of co-metabolism substrates were studied and compared. The experimental results showed that the presence of co-metabolism substrates significantly increased xanthate degradation. The degradation rate of butyl xantbate was 43.1% after shaking culture for 72 h at 25 ℃ with butyl xanthate as the sote source of carbon and energy. While the presence of additional carbon source such as glucose,sucrose and amylum （0.20 g/L） increased the degradation rate of butyl xanthate to 65.2%, 59.4% ,56.4% under the same biodegradation condition,and the coexistence of nitrogen source （peptone） and carbon source （glucose） increased the butyl xanthate degradation to 73.5%. The variation of intermediates products during butyl xanthate biodegradation were detected, with help of which the biodegradation pathway of butyl xanthate was analyzed. The co-metabolism shortened adaptation stage of microbial from original of 36 h to 24 h,so the co-metabolism might be one of the effective ways to improve the biodegradability of butyl xanthate.

关 键 词：丁基黄药 共代谢 生物降解 

分 类 号：X703[环境科学与工程—环境工程]