5-HT对大鼠新鲜分离DRG神经元GABA激活电流的调制作用  

INHIBITORY EFECT OF 5 HT ON GABA ACTIVATED CURRENTS IN FRESHLY ISOLATED RAT DRG NEURONS

作　　者：李韶[1,2] 崔存德 关兵才[1,2] 李之望 胡宏镇[1,2] 

机构地区：[1]同济医科大学实验中心 [2]滨州医学院生理教研室

出　　处：《中国应用生理学杂志》1998年第4期368-377,共10页Chinese Journal of Applied Physiology

摘　　要：本实验在新鲜分离的大鼠背根神经节（ＤＲＧ）细胞上应用全细胞膜片钳技术，观察５－ＨＴ对ＧＡＢＡ激活电流的调制作用。结果发现，绝大多数细胞（４９／５４）对ＧＡＢＡ（１０－８～１０－３ｍｏｌ／Ｌ）产生浓度依赖性的内向电流，并有明显的去敏感现象。在多数细胞预加５－ＨＴ（１０－７～１０－４ｍｏｌ／Ｌ），ＧＡＢＡ激活电流受到明显抑制，抑制率分别为３．０％，１３．５％，１９．７％，２４．７％，５９．１％。５－ＨＴ（１０－７～１０－４ｍｏｌ／Ｌ）本身在部分细胞（２４／４０）可引起浓度依赖性的内向电流，部分细胞（１２／４０）未检测到膜电流，少数细胞（４／４０）可引起微弱的外向电流。此结果提示５－ＨＴ可能作用于初级传入终末。The whole cell patch clamp technique was used to explore the effect of 5 HT on the GABA activated currents in freshly isolated DRG neurons. The results were as follows: Application of GABA (10 -8 ￣10 -3 mol/L) could induce concentration dependent inward currents with desensition in the majority of cells(49/54);Preapplication of 5 HT(10 -8 ￣10 -3 mol/L)could inhibit the GABA activated inward currents in most cells, the inhibitory rates being 3.0%, 13.5%,19.7%, 24.7%,59.1% respectively; application of 5 HT (10 -7 ￣10 -3 mol/L) alone could induce concentration-dependent inward currents in some cells (24/40), slight outward currents in several other cells(4/40), and no current in the rest (12/40). The results suggested 5 HT could modulate the GABA induced presynaptic inhibition in primary afferent terminals.

关 键 词：5-HT GABA 背根神经节 全细胞膜片钳 激活电流 

分 类 号：R338.8[医药卫生—人体生理学]