邻苯二酚偶氮苯衍生物-苯硼酸体系对糖的识别  

Recognization for Saccharide Based on Catechol Azobenzene Derivatives and Phenylboric Acid

作　　者：吴芳英[1] 朱文兵[1] 胡美华[1] 

机构地区：[1]南昌大学化学系,江西南昌330031

出　　处：《南昌大学学报（理科版）》2010年第1期85-89,共5页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金资助项目(No.20965006);江西省教育厅资助项目(GJJ09040)

摘　　要：合成了4种邻苯二酚偶氮苯衍生物,利用邻二酚与糖中邻羟基相似的结构特点,以苯硼酸为中介利用竞争结合作用建立糖的识别方法。为模拟生物体中性环境,在pH7.4 KH2PO4-NaOH缓冲溶液中,以邻苯二酚偶氮苯衍生物为指示剂,与苯硼酸组成二组分体系,糖与指示剂竞争结合苯硼酸使指示剂游离出来,产生明显的光谱变化,据此建立糖的识别方法。考察了葡萄糖、山梨醇、D-半乳糖、D-甘露糖、D-果糖和D-阿拉伯糖对上述二组分体系光谱的影响,结果显示:该体系对山梨醇和D-果糖有较好的光谱响应。Four catechol azobenzene derivatives were synthesized. Based on the structural similarity between catechol and adjacent dihydroxy of saceharide, phenylboric acid was utilized as an intermediary agent to build a method to recognize the saecharide. An azocateehol was used as indicator to form a two - component system with phenylboric acid. Then, saceharide bound with phenylborie acid, and the indicator was free from the system. A remarkable spectrum change came into being. Consequently, saccharide could be sensed in pH 7.4 KH2PO4 -NaOH buffer. Experiment results indicated that the system showed better spectral response to sorbitol and D -fructose than other four studied monosaccharides such as glucose, D -galaetose, D -mannitol and D- arabinose.

关 键 词：邻苯二酚偶氮苯衍生物 苯硼酸 单糖 紫外可见分光光度法 

分 类 号：O629.112[理学—有机化学]