百日咳杆菌有效免疫原的发酵培养  

Fermental cultivation of Bordetella pertussis immunogens

作　　者：秦力[1] 杨晓明[1] 鲁劲民 刘志源[1] 陈爱荣[1] 范晓昌 何长民[1] 

机构地区：[1]卫生部兰州生物制品研究所

出　　处：《微生物学免疫学进展》1998年第4期41-45,共5页Progress In Microbiology and Immunology

摘　　要：为扩大生产，采用５００立升发酵罐培养无细胞百日咳菌苗，发现随着培养时间的延续，细胞浓度增高，培养液的ｐＨ值上升，ＰＴ、ＦＨＡ活性、血凝效价逐渐增加、Ｏ２溶压下降、ＣＯ２溶压上升。ｐＨ值达８２时，ＰＴ活性最高为３００ＥＵ／ｍｌ，较现用扁瓶培养方法高５倍。ｐＨ值继续上升时，ＰＴ活性开始下降。ＦＨＡ活性及血凝效价具有相似的变化。通过测定培养液ｐＨ值以确定收获时间，可获得富含ＰＴ、ＦＨＡ。Bordetella pertussis was cultivated in 500 liter fermentor.We discovered that as the cultural time prolonged,the bacteria concentration pH value,PT.FHA and agglutinogen activity of culture supernatant gradually increased and the dissoloved O 2 pressure decreased while the dissolved CO 2 pressure rose.When culture pH reached 8.2,the activity of the main immunogen PT was 5 times (300EU/ml) higher than did by conventional cultivation method.The activity of PT began to decrease while culture supernatant pH still increased.The activity of FHA and agglutinogen had the same results.The time of harvest can be decided by measuring culture pH value and the culture supernatant containing rich PT and FHA can be gained.

关 键 词：发酵培养 丝状血凝素 百日咳毒素 

分 类 号：R378.42[医药卫生—病原生物学]