志贺氏毒素B亚单位的分离纯化及其多克隆抗体的制备  

Isolation and purification of StxB and production of polyclonal antisera against StxB

作　　者：冯尔玲[1] 韩照中[1] 王恒梁[1] 苏国富[1] 

机构地区：[1]军事医学科学院生物工程研究所

出　　处：《微生物学免疫学进展》1998年第4期55-57,共3页Progress In Microbiology and Immunology

摘　　要：从高效表达志贺氏毒素Ｂ亚单位（ＳｔｘＢ）的工程菌株ＤＨ５α／ｐＳＵ１０８分离纯化了ＳｔｘＢ，并用它制备了多克隆抗体。ＥＬＩＳＡ试验表明抗ＳｔｘＢ抗血清的滴度达１×１０４。Ｗｅｓｔｅｒｎｂｌｏｔ结果显示该抗血清能与ＳｔｘＢ发生特异反应。In this study StxB was isolated and purified from DH5α/pSU108 which could express StxB at high level.Then polyclonal antisera against StxB was prepared.ELISA indicated that the titre of this antisera achieved 1×10 -4 .Western blot showed that this antisera could react specifically with StxB.This study played the basis for the research of StxB immunoprotection and the construction of Shigella dysenteriae type 1 candidate vaccine.

关 键 词：志贺氏毒素B亚单位 分离和纯化 抗血清 

分 类 号：R378.25[医药卫生—病原生物学] R996.1[医药卫生—基础医学]