Ucf-101对窒息新生大鼠肾小管上皮细胞凋亡的影响  

Effect of Ucf-101 on Apoptosis of Tubular Epthelial Cells in Postasphyxial Neonatal Rats

作　　者：花兵[1] 董文斌[1] 李清平[1] 冯志强[2] 余鸿[3] 翟雪松[1] 雷小平[1] 

机构地区：[1]泸州医学院附属医院新生儿科,四川泸州646000 [2]泸州医学院生理学教研室,四川泸州646000 [3]泸州医学院组织胚胎学教研室,四川泸州646000

出　　处：《实用儿科临床杂志》2010年第6期409-411,共3页Journal of Applied Clinical Pediatrics

基　　金：四川省杰出青年学科带头人培养基金(04ZQ026-033);四川省科技厅应用基础项目(2008JY0015);<中华儿科杂志>第1届双鹤珂立苏科研基金

摘　　要：目的研究Omi/HtrA2丝氨酸蛋白酶特异性抑制剂Ucf-101对窒息新生大鼠肾小管上皮细胞凋亡的影响。方法选择7～10日龄Wistar大鼠45只,其中30只置于容积为55mL缺氧瓶中(内装0.005kg钠石灰),待动物安静后,塞紧瓶塞,制作新生大鼠常压窒息模型。对照组模拟该过程,不塞瓶塞。造模后的大鼠随机分为窒息组(15只)、Ucf-101组(15只)、对照组(15只)。Ucf-101用量为1.5μmol.kg-1,在窒息前10min腹腔注射。窒息组及对照组在相同时间点注射等量9g.L-1盐水。窒息组及Ucf-101组密闭30min后复氧,在复氧2h、24h、48h时断颈处死后取左肾,制作石蜡切片,采用原位细胞凋亡TUNEL法检测窒息后2h、24h、48h新生大鼠左肾凋亡细胞的形态及数量变化。结果新生大鼠窒息后不同时间点肾脏皮质、髓质均有凋亡细胞分布,多数为肾小管上皮细胞,细胞核呈棕褐色,形态不一;窒息组复氧后2h,肾皮质开始出现大量的TUNEL染色阳性细胞,24h凋亡细胞数达高峰,48h凋亡细胞数有所下降;窒息组各时间点凋亡细胞数与对照组比较均显著增加(Pa<0.05)。对照组凋亡细胞数未见明显增加,各时间点无统计学差异。与窒息组比较,Ucf-101组各个时间点凋亡细胞数均明显减少(Pa<0.01)。结论窒息后新生大鼠肾细胞凋亡显著增高,且具有时间依赖性,同时凋亡参与肾损伤过程,Ucf-101能明显抑制凋亡发生。细胞凋亡是窒息后新生大鼠肾损伤的重要机制。Objective To study the effect of Ucf-101,as an Omi/HtrA2 inhibitor,on tubular epthelial cell apoptosis induced by postasphyxial injury in neonatal rats.Methods Forty-five cases of 7-10 day-old Wistar rats were selected,and 30 cases of them were placed in hypoxic bottle of 55 mL volume（internal 0.005 kg sodium lime）,when they were quiet,the stopper were plugged.The rats in normal control group（n=15）to simulate the process did not have the plugged stopper.It was the asphyxia model of atmospheric pressure in neonatal rats.Then the rats were randomly divided into asphyxia group（n=15）and Ucf-101 group（n=15）.The dosage of Ucf-101 was 1.5 μmol·kg-1,intraperitoneally injected 10 min before asphyxia.The rats in asphyxia group and control group were injected with saline at the same time.Then the stopper after 30 min of asphyxia was opened.The rats were killed at 2 h,24 h,48 h after asphyxia,and the left kidney was taken for paraffin sections.Terminal deoxynuleotidyl-mediated nick end labeling（TUNEL）was used to detect the apoptosis of cell at 2 h,24 h,48 h after asphyxia.Results TUNEL-positive cells stained in yellow were seen in the whole kidney,and positive products located in the nucleus.The apoptotic cells were mainly tubular epithelial cells.A few of TUNEL-positive cells were observed in the cortex and medulla of kidney at 2 h and reached the peak at 24 h after asphyxia,and the apoptotic cells in every time point of postasphyxial group were significantly increased compared with control group（Pa〈0.05）.The number of TUNEL-positive cells were decreased in the kidney samples of the Ucf-101 group compared with postasphyxial group（Pa〈0.01）.Conclusions Renal injury after asphyxia induces the apoptosis of renal cells,which is time-dependent.Ucf-101 can inhibit apoptosis.Apoptosis is an important post-asphyxial mechanism of renal injury.

关 键 词：窒息 肾损伤 Ucf-101 细胞凋亡 

分 类 号：R722.1[医药卫生—儿科]