光周期对飞虱虫疠霉离体培养的生物量及其产孢节律的影响  

THE EFFECT OF PHOTOPERIODS ON THE GROWTH AND SPORULATION OF THE ENTOMOPHTHORALEAN FUNGUS, PANDORA DELPHACIS

作　　者：徐均焕[1] 冯明光[1] 

机构地区：[1]浙江农业大学生物科学系

出　　处：《菌物系统》1998年第4期349-355,共7页Mycosystema

基　　金：国家杰出青年科学基金;浙江省自然科学基金

摘　　要：飞虱虫疠霉（Ｐａｎｄｏｒａｄｅｌｐｈａｃｉｓ）是侵染飞虱和叶蝉等重要作物害虫的昆虫病原真菌。通过在液体及平板培养中研究光周期对菌丝及菌落生长量、产孢节律和产孢量的影响，作者发现光照对液培菌丝的营养生长无显著影响，但长光照液培菌丝的产孢延迟并且产孢量减少，而全黑暗液培菌丝产孢早且产孢量大。但是，光照明显促进全黑暗下液培菌丝的产孢。在平板培养中，长光照能促进菌落生长和产孢；半光照半黑暗虽有利菌落生长，但产孢量很低；光照９ｈ和１８ｈ产孢量较大，但菌落生长较小。无论液体还是平板培养，飞虱虫疠霉均生长良好，但建议在全黑暗条件下进行液体培养，在长光照下进行平板培养。The entomophthoralean fungus, Pandora delphacis, is an important pathogen infecting delphacids and cicadellids. In the present study, the effect of the photoperiods, ranging from 0 to 24 h light at 3 h intervals, on the growth and sporulation patterns of the fungus was evaluated using liquid and plate cultures. The resulted data showed that the photoperiods affected insignificantly on the amount of the fungal biomass during the liquid culture at 28℃. The fungal masses porduced at long photoperiods sporulated later and less than those produced at dark and short photoperiods during the incubation on agar plates at 24℃ and 12L∶12D. When incubated on agar plates at varying photoperiods, the fungal masses obtained from the liquid culture at dark sporulated least at 12L∶12D and 21L∶3D but with less variation among other photoperiods. During the plate culture, long photoperiods apparently favored the colony growth and sporulation; half dark and half ligh resulted in fast colony growth but less sporulation whereas 9 h and 18 h light led to more sporulation but slower colony growth. The results showed that P. delphacis was easily grown either in liquid culture or on agar plates. Entire darkness was recommended for liquid culture and long photoperiods for plate culture.

关 键 词：飞虱虫疣霉 虫霉 光周期 产孢 培养 

分 类 号：Q949.323.4[生物学—植物学]