电鳐β-蝮蛇毒素结合蛋白的抽提及其与毒素结合性质的研究  

SOLUBILIZATION OF β AGKISTRODOTOXIN BINDING PROTEIN FROM Torpedo ELECTRIC ORGAN AND STUDIES ON ITS BINDING PROPERTIES WITH THE TOXIN

作　　者：王银[1,2] 徐科[1,2] 沈国光 杜晓燕[1,2] 周元聪 

机构地区：[1]中国科学院上海生理研究所 [2]中国科学院上海生物化学研究所

出　　处：《Zoological Research》1998年第6期429-433,共5页动物学研究（英文）

基　　金：国家自然科学基金

摘　　要：以加州电鳐（Ｔｏｒｐｅｄｏｃａｌｉｆｏｒｎｉｃａ）电器官为材料，探索了用去垢剂ＴｒｉｔｏｎＸ－１００增溶抽提β－蝮蛇毒素（βａｇｋｉｓｔｒｏｄｏｔｏｘｉｎ，βＡｇＴＸ）结合蛋白的合适条件，建立了此结合蛋白活性的检测方法，并分析了该结合蛋白与同位素１２５Ｉ标记βＡｇＴＸ的结合性质。结果显示这种电器官中存在相对含量较高的毒素结合位点，其密度为１５８０ｆｍｏＬ／ｍｇ蛋白质，此结合作用的平衡解离常数ＫＤ值为５５×１０－９ｍｏｌ／Ｌ。去垢剂ＴｒｉｔｏｎＸ－１００可以有效地将该结合蛋白从电器官组织膜上增溶下来，抽提效率接近５０％，为分离、纯化此结合蛋白建立了基础。It was infered that β agkistrodotoxin binding protein is presumably a novel presynaptic functional protein involved in neurotransmitter release.This paper reported studies on solubilization of β agkistrodotoxin binding protein from Torpedo californica electric organ, a tissue rich in synapses.The binding assay of this protein was established using a PEG precipitation method, and its binding properties with 125 I labelled β AgTX was preliminarily determined.The results showed that the density of β AgTX binding sites in this tissue is relatively high (much higher than that of rat brain).B max is 1580 fmol/mg protein and K D value is 5 5×10 -9 mol/L. Triton X 100 was proved an effective detergent for the extraction experiment with a yield of about 50%.Thus a hopeful beginning for isolation and purification of β agkistrodotoxin binding protein was accomplished.

关 键 词：β蝮蛇神经毒素 结合蛋白 增溶抽提 电鳐电器官 

分 类 号：Q510.3[生物学—生物化学] R996.3[医药卫生—毒理学]