纳米火棉胶膜自组装细胞色素c的直接电化学研究  

Direct electrochemistry of cytochrome c immobilized in nanoporous collodium

作　　者：秦玉华[1] 周立恒[1] 王岩[1] 马丽[1] 王胜天[2] 

机构地区：[1]东北电力大学化学工程学院,吉林吉林132012 [2]东北师范大学化学学院,吉林长春130024

出　　处：《东北师大学报（自然科学版）》2010年第1期93-97,共5页Journal of Northeast Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30800191)

摘　　要：用纳米火棉胶膜将细胞色素c固定在玻碳电极表面,制备了细胞色素c-火棉胶膜修饰电极.吸附在火棉胶膜上的细胞色素c可以与电极发生直接电子传递.在pH=7.0的0.1mol/LPBS缓冲溶液中可得到一对准可逆的细胞色素c的血红素辅基Fe(Ⅲ)/Fe(Ⅱ)电对氧化还原峰,实验求得细胞色素c异相电子传递速率常数k0为65.4μm/s.进一步考察了扫速、溶液pH值等因素对细胞色素c电子传递的影响,并用电化学阻抗法研究了修饰电极的电化学行为.Cytochrome c was immobilized on glass carbon electrode by collodium.The cytochrome c adsorbed in the collodium film undergoes fast direct electron-transfer reactions,corresponding to FeⅢ＋e-→FeⅡ in 0.1 mol/L PBS buffer （pH = 7.0）.The heterogeneous electron transfer rate constant （k0） of cytochrome c was evaluated to be ca.65.4 μm/s.The effect of scan rate,pH and concentration of buffers on the electrochemical behaviors of cytochrome c is studied in detail.Electrochemical property of cytochrome c on modified electrode is investigated by electrochemical impedance spectroscopy.

关 键 词：细胞色素C 火棉胶 直接电化学 

分 类 号：O657.1[理学—分析化学] O152.3[理学—化学]