Total Fractionation and Analysis of Polysaccharides from Leaves of Panax ginseng C.A. Meyer  

作　　者：NI Xiu-zhen WANG Bing-qing ZHI Yuan WEI Ning-ning ZHANG Xu LI Shan-shan TAI Gui-hua ZHOU Yi-fa ZHAO Ji-ming 

机构地区：[1]School of Life Sciences, Northeast Normal University, Changchun 130024, P. R. China [2]School of Life Sciences, Changchun Normal University, Changchun 130032, P. R. China

出　　处：《Chemical Research in Chinese Universities》2010年第2期230-234,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(Nos.30570417 and 30973857);the Natural Science Foundation of Jilin Province(No.20070710);the Natural Science Foundation of Changchun, China(Nos.2009081 and 09SF03)

摘　　要：The water-soluble polysaccharides isolated from the leaves of Panax ginseng C. A. Meyer were totally fractionated into one neutral fraction（GLPN-1）, six acidic fractions（GLPN-2, GLPA-1a, GLPA-1b, GLPA-1c, GLPA-2 and GLPA-3） by a combination of ethanol precipitation, ion-exchange and gel permeation chromatography. All the fractions were analyzed by determining monosaccharide composition, molecular weight distribution. The resuits show that GLPN-1 was mainly composed of Gal（38.1%） and Ara（33.6%） and presumed to be an arabinogalactan. GLPA-1a, GLPA-1b and GLPA-2 mainly contained Gal, Ara, Rha and GalA. The amount of Gal and Ara acounted for over 50% of total sugar and the amount of Rha was more than 10%. The molar ratios of Rha/GalA in GLPA-1a, GLPA-1b and GLPA-2 were 1：1, 1：2 and 1：2.7, respectively, indicating that they belong to type-1 rhamnogalacturonan （RG-1）-rich pectin. GLPA-1c was mainly composed of GalA（78%）, belonging to homogalacturonan（HG）-rich pectin. GLPA-3 might be a glycoprotein.The water-soluble polysaccharides isolated from the leaves of Panax ginseng C. A. Meyer were totally fractionated into one neutral fraction（GLPN-1）, six acidic fractions（GLPN-2, GLPA-1a, GLPA-1b, GLPA-1c, GLPA-2 and GLPA-3） by a combination of ethanol precipitation, ion-exchange and gel permeation chromatography. All the fractions were analyzed by determining monosaccharide composition, molecular weight distribution. The resuits show that GLPN-1 was mainly composed of Gal（38.1%） and Ara（33.6%） and presumed to be an arabinogalactan. GLPA-1a, GLPA-1b and GLPA-2 mainly contained Gal, Ara, Rha and GalA. The amount of Gal and Ara acounted for over 50% of total sugar and the amount of Rha was more than 10%. The molar ratios of Rha/GalA in GLPA-1a, GLPA-1b and GLPA-2 were 1：1, 1：2 and 1：2.7, respectively, indicating that they belong to type-1 rhamnogalacturonan （RG-1）-rich pectin. GLPA-1c was mainly composed of GalA（78%）, belonging to homogalacturonan（HG）-rich pectin. GLPA-3 might be a glycoprotein.

关 键 词：Ginseng leave POLYSACCHARIDE FRACTIONATION RG-I domain 

分 类 号：TQ028.8[化学工程] TS275.5[轻工技术与工程—农产品加工及贮藏工程]