隆安县乙肝病毒无症状携带者HBV前S基因缺失突变的研究  

Study of Hepatitis B Virus Pre-S Deletion Mutations in HBsAg Asymptomatic Carriers from Long'an County

作　　者：王学燕[1] 李国坚[2] 董柏青[1] 陈钦艳[1] 方孔雄[3] 杨进业[1] 黄坚[3] 韦少超[3] 方钟燎[1] Caroline A Sabin Tim J Harrison 

机构地区：[1]广西壮族自治区疾病预防控制中心,南宁市530028 [2]广西壮族自治区卫生厅,南宁市530021 [3]广西隆安县疾病预防控制中心,隆安县532700 [4]英国伦敦大学学院

出　　处：《广西医学》2010年第2期129-133,共5页Guangxi Medical Journal

基　　金：英国Welcome基金会资助项目(072058/Z/03/Z)

摘　　要：目的了解肝癌高发区隆安县乙型肝炎病毒(HBV)前S基因缺失突变株的流行情况。方法HBV表面抗原(HBsAg)无症状携带者从我们隆安县研究队列中选择,用套式聚合酶链反应对研究对象血清HBV前S基因扩增和序列分析。结果在66例标本中9例出现前S基因缺失突变,占13.6%(9/66)。9例缺失突变均为框内突变,其中7例突变发生在或涉及前S2区的5'末端。男6例、女3例出现前S基因缺失突变,但男女间突变率(12.0%,6/50与18.8%,3/16)差异无统计学意义(χ2=0.709,P>0.10);前S基因缺失突变率在基因型B、基因型C和重组基因型之间差异无统计学意义(χ2=0.002,P>0.95)。结论肝癌高发区隆安县居民HBV无症状携带者前S基因缺失突变率较高,这种突变与肝癌的关系值得进一步探讨。Objective To understand the prevalence of hepatitis B virus（HBV）pre-S deletion mutations in HBsAg asymptomatic carriers from Long＇an county.Methods All subjects were selected from HBsAg asymptomatic carriers of the Long＇an cohort,and nested RT-PCR was used to amplify and sequence the sera pre-S region of HBV.Results 9 subjects selected had pre-S deletions,counting for 13.6%（9/66）.All deletions were in-frame and seven of them occurred in or involved the 5＇ terminus of pre-S2.The pre-S deletion mutation rate between male and female was not significantly different（12%,6/50 for male and 18.8%,3/16 for female,χ^2=0.709,P〉0.10）.There was also no significant difference in the pre-S deletions mutation rate among genotype B and C and the recombinant（χ^2=0.002,P〉0.95）.Conclusion The HBV pre-S deletion mutation rate of HBsAg asymptomatic carriers in Long＇an county is relatively high.The relationship between the mutations and the development of hepatocellular carcinoma is worth further investigation.

关 键 词：乙型肝炎病毒 表面抗原无症状携带者 前S基因缺失突变 隆安县 

分 类 号：R512.26[医药卫生—内科学]