对细菌中2-氧化戊二酸依赖型加氧酶系存在性的分析  

The analysis of the probability of 2-oxoglutarate-dependent dioxygenase family in bacteria

作　　者：刘守寒[1] 唐天元[1] 刘忠于[1] 杨毅[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2010年第2期361-365,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(30671165,2007AA021304-1)

摘　　要：2-酮戊二酸,铁离子和维生素C是氧化戊二酸依赖型加氧酶辅助因子.有的细菌缺少维生素C,因此可能不含维生素C独立体酶,为了确定细菌中是否含有这种酶系,本文首先利用生物学软件ClustalX,PHYLIP,MEME/MAST和HMMER在植物和动物体内验证这个酶系的系统发育关系和保守序列,然后利用那些保守序列可以用来检测细菌是否含有这种酶.分析表明细菌中确实含有2-氧化戊二酸依赖型加氧酶酶系.The 2-oxoglutarate-dependent dioxygenase （2ODD） family has a character requiring 2-oxoglutarate, iron ion and ascorbate as essential co-factors. The 2-oxoglutarate and iron-binding sites must be conserved in this super family. Bacteria probably lack ascorbate （Vitamin C） ; hence, they may not have the ascorbate-dependent forms of enzymes. To identify if it exist in bacteria, software predicting measures identified relationship and conserved sequences of this family in plants and animals were used, and then those conserved sequences can be used to determine if bacteria have this family enzyme.

关 键 词：进化树 生物信息学分析 保守序列 细菌 2-氧化戊二酸依赖型加氧酶 

分 类 号：Q81[生物学—生物工程]