黑腹果蝇抗真菌肽基因Drs的真核可溶性表达  

Soluble expression of the antifungal peptide genes Drs of Drosophila melanogaster in the eukaryote

作　　者：李征[1] 黄玉兰[1] 何特[1] 李明雄[1] 张杰[1] 杨志荣[1] 罗璠[1,2] 

机构地区：[1]四川大学生命科学学院资源微生物与微生物技术四川省重点实验室,成都610064 [2]西南民族大学生命科学与技术学院,成都610041

出　　处：《四川大学学报（自然科学版）》2010年第2期371-376,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省科技攻关项目(07KJT20-20)

摘　　要：将黑腹果蝇(Drosophila melanogaster)抗真菌肽基因Drosomycin(Drs)克隆到pPICZα-A载体中,构建分泌型表达载体pPICZα-A-Drs,转化宿主菌Pichia pastoris X-33.在AOX1(醇氧化酶)启动子调控下,抗真菌肽DRS成功表达,其分子量约为5 kD.抑菌试验显示,DRS对供试真菌有明显的抑菌活性.采用考马斯亮蓝法测定抗真菌肽的具体表达量,并优化了诱导条件.The Drosophila melanogaster antifungal peptide gene Drosomycin （Drs）, was expressed in Pichia pastoris X-33 transformed by the recombinant expression vector pPICZα-A-Drs, which was combined by vector pPICZα-A and Drs. This resulting vector pPICZα-A-Drs contained α-Factor under the control of the alcohol oxidase 1 promoter. Following induction with methanol,the DRS peptide was synthesized with a molecular weight of 5.0 kD in a soluble form. Antifungal assay showed that the DRS had a obvious inhibitive effect on tested fungi. The amount of expression product DRS were determined by the coomassie blue staining,and the conditions of the expression were optimized.

关 键 词：果蝇 抗真菌肽 分泌表达 毕赤酵母 

分 类 号：Q78[生物学—分子生物学]