环孢素A对人类骨髓间充质干细胞增殖活性的影响  

Effect of cyclosporin A on the proliferation activity of mesenchymal stem cells

作　　者：王雁玲[1] 乔振华[1] 仇丽霞[2] 贡蓉 贾清云[1] 褚德勤[4] 牛勃[5] 

机构地区：[1]山西医科大学第二医院血液科,太原030001 [2]山西医科大学卫生统计教研室 [3]山西省第二人民医院血液科 [4]山西省肿瘤医院门诊部 [5]首都儿科研究所生物技术室

出　　处：《白血病．淋巴瘤》2010年第3期169-171,共3页Journal of Leukemia & Lymphoma

摘　　要：目的 探讨环孢素A（CsA）对人类骨髓间充质干细胞（MSC）增殖活性的影响,为研究CsA对骨髓增牛异常综合征（MDS）患者骨髓MSC增殖的影响提供依据.方法 采用四甲基偶氮唑盐（MTT）微量酶反应比色法,经2.5×10^4、2.5×10^3、2.5×10^2、2.5×10 ng/μl CsA刺激后检测对照组和MDS患者骨髓MSC的吸光度（A值）.结果 CsA不同质量浓度间骨髓MSC增殖效应差异无统计学意义（P〉0.05）;在（2.5×10～2.5×10^4）ng/μl质量浓度范围内,未观察到CsA有细胞毒性作用;MDS患者骨髓MSC增殖效应高于健康对照（P〈0.01）.CsA质量浓度与组别问交瓦作用差异无统计学意义（P〉0.05）.不同质量浓度CsA处理组与对照组骨髓MSC增殖效应差异无统计学意义.结论 CsA对MDS患者骨髓MSC和健康患者MSC增殖影响不明显.Objective To provide data for reference on the impact of cyclosporin A （CsA） on the proliferation of the bone marrow mesenchymal stem cells in MDS patients through the investigation of the impact of cyclosporin A on human bone marrow mesenchymal stem cell proliferation. Methods The absorption rates of the bone marrow mesenchymal stem cells in the control group and the MDS patient group were determined by using the tetrazolinm salt （MTT） micro-colorimetric enzyme reaction. The concentrations of cyclosporine A are 2.5×10^4 ng/μl, 2.5×10^3 ng/μl, 2.5×10^2 ng/μl and 2.5×10ng/μl respectively. Results There is no significant difference between the each result by using different concentrations of CsA., which indicates the impact of CsA on the growth of mesenchymal stem cells is not significant（P 〉0.05）. In the serial of concentrations mentioned, no cytotoxicity of CsA is observed. However, there is difference between the selected indicators of the control group and the patient group （P 〈0.01）, and the value of the MDS patient group is higher than that of the control group. There is no statistic difference between the concentration of CsA and the data obtained from interactions between different groups （P 〉0.05）. There is no significant difference between the absorption rates of the group treated by CsA of every concentration and the corresponding control group. Conclusion The impact of CsA on the bone marrow mesenchymal stem cell proliferation is significant in neither healthy people nor MDS patients.

关 键 词：骨髓增生异常综合征 环孢素A 间质干细胞 细胞增殖 MDS 

分 类 号：R551.3[医药卫生—血液循环系统疾病]