MRL/lpr小鼠肾组织胸腺和活化调节趋化因子表达检测  

Expression of Thymus and Activation-regulated Chemokine in the Kidney of MRL/lpr Mice

作　　者：刘海娜[1] 吴春玲[1] 肖卫国[1] 

机构地区：[1]中国医科大学附属第一医院风湿免疫科,沈阳110001

出　　处：《中国医科大学学报》2010年第3期171-173,共3页Journal of China Medical University

基　　金：辽宁省科学技术计划资助项目(2007225005-6)

摘　　要：目的研究胸腺和活化调节趋化因子(TARC)在红斑狼疮小鼠肾组织中的表达与作用。方法16周龄MRL/lpr小鼠和BALB/c小鼠各4只,比较两组小鼠24h尿蛋白,并用逆转录-聚合酶链反应(RT-PCR)技术和蛋白印迹的方法检测两组小鼠肾组织中TARC mRNA及蛋白表达情况。结果MRL/lpr小鼠24h尿蛋白高于BALB/c小鼠(P<0.05);MRL/lpr小鼠肾组织TARC mRNA表达较BALB/c小鼠明显升高(P<0.05);MRL/lpr小鼠肾组织中可检测出TARC蛋白条带,而BALB/c小鼠肾脏组织中未能测得。结论TARC在红斑狼疮小鼠肾脏组织中表达增高,并可能参与介导小鼠狼疮性肾炎的发病。Objective To study the expression of thymus and activation-regulated chemokine（TARC）in the kidney of MRL/lpr mice and to elucidate the molecular pathogenesis of lupus nephritis. Methods The 24-hour urine protein of 4 MRL/lpr mice of 16 weeks of age and 4 age-and sex-matched BALB/c mice was measured. The expressions of TARC mRNA and protein in the kidneys of MRL/lpr mice and BALB/c mice were detected by reverse transcription polymerase chain reaction and Western blot,respectively. Results The 24-hour urine protein and the expression level of TARC mRNA were significantly higher in MRL/lpr mice than in BALB/c mice （P 0.05）. The expression of TARC protein was detectable only in MRL/lpr mice. Conclusion The expression of TARC increases in the kidney of mice with systemic lupus erythematosus,suggesting that TARC may be involved in lupus nephritis in mice.

关 键 词：胸腺和活化调节趋化因子 红斑狼疮 系统性 狼疮性肾炎 

分 类 号：R593.24[医药卫生—内科学]