HPLC法测定降脂宁调脂抗氧化有效部位中4种蒽醌类成分的含量  

Quantitative determination of 4 anthraquinone constituents in effective fractions of Jiangzhining by HPLC

作　　者：徐冬艳[1] 刘斌[1] 王伟[1] 石任兵[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《北京中医药大学学报》2010年第3期203-206,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：十一五国家科技支撑计划资助项目(No.2006BAI08B03-04);北京市自然科学基金资助项目(No.7061002);国家自然科学基金资助项目(No.90409011)

摘　　要：目的建立降脂宁有效部位中4种蒽醌类成分橙黄决明素、大黄素、大黄酚、大黄素甲醚的HPLC含量测定方法。方法采用SunFire C_(18)柱(150 mm×4.6 mm,5μm),柱温30℃,流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,流速为1.0 mL/min,检测波长285 nm。结果橙黄决明素、大黄素、大黄酚、大黄素甲醚平均回收率分别为98.69%、99.97%、97.98%和99.20%,RSD分别为2.20%、1.18%、2.27%和1.15%。结论3批样品测定结果表明,该方法简便、准确,可用于降脂宁有效部位中4种蒽醌类成分的含量测定。Objective To establish a method for quantitative determination of four anthraquinone constituents,including aurantio-obtusifolin,emodin,chrysophanol and physcion,in the effective fraction of Jiangzhining by HPLC.Methods The procedure of HPLC was performed on the chromatographic column of SunFire C18（150 mm×4.6 mm,5μm） at the temperature of 30℃.The mobile phase in the gradient elution was acetonitrile-phosphoric acid solution（0.1%）,the flow rate was 1.0 mL/min,and the detection wavelength was 285 nm.Results The average recovery of aurantio-obtusifolin,emodin, chrysophanol or physcion was,respectively,98.69%,99.97%,97.98%and 99.20%,and RSD was 2.20%,1.18%,2.27%and 1.15%.Conclusion The determination results of three batches of samples showed that the method was easy and accurate,which can be used in the quantitative determination of four anthraquinone constituents in the effective fraction of Jiangzhining.

关 键 词：降脂宁 橙黄决明素 大黄素 大黄酚 大黄素甲醚 含量测定 

分 类 号：R284.1[医药卫生—中药学]