两种精子优化法对精子动态、形态的影响比较  

Study on the motility and morphology changes of the spermatozoa after treatment of two preparation methods

作　　者：刘锦宏[1] 毕慧玲[1] 李锐[1] 韩丽娟[1] 刘俊恒[1] 

机构地区：[1]长治医学院附属和平医院生殖遗传科,山西046000

出　　处：《齐齐哈尔医学院学报》2010年第3期343-344,共2页Journal of Qiqihar Medical University

摘　　要：目的探讨上游法和密度梯度离心法处理精液前后精子的动态、形态变化。方法对78例精液标本分别行上游法和密度梯度离心法优选,处理前后均用伟力精子分析系统和Diff-Quick快速染色法进行精子动态、形态分析。结果经两种方法优化后精子密度、活率、活力都有显著提高(P<0.05),同时精子畸形率有显著降低(P<0.05)。结论两种精子优化法均可以提高精子质量,有助于IUI(宫腔内人工授精)助孕患者达到受孕目的 。Objective To investigate the motility and morphology changes of the spermatozoa before and after the treatments of swimming up and density gradient centrifugation procedure. Methods 78 semen specimens were selected by swimming up and density gradient centrifugation procedure separately. Before and after that,analyzed spermatozoa kinesis parameters by the WLJY--9000 color semen analysis system,detected spermatozoa morphology with Diff-Quick staining method. Results These two methods could increase the motility and reduce the malformation rates of the spermatozoa markedly. Conclusions These two methods can increase the quality of the spermatozoa and be helpful for infertility patients.

关 键 词：精子 上游 密度梯度离心 Diff—Quick快速染色 

分 类 号：R996[医药卫生—毒理学]