彩色甜椒基因组DNA提取方法的优化  

Optimization of Extraction Methods for Genomic DNA from Color Sweet Pepper

作　　者：刘维侠[1] 曹振木[1] 王秀全[2] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所/农业部热带作物种质资源利用重点开放实验室,海南儋州571737 [2]中国热带农业科学院橡胶研究所,海南儋州571737

出　　处：《热带农业科学》2010年第2期19-23,共5页Chinese Journal of Tropical Agriculture

基　　金：中央级公益性科研院所基本科研业务费专项(No.PZS057);海南省自然科学基金项目(No.807038)

摘　　要：以不同颜色的甜椒为试验材料,采用CTAB法、SDS法、ROSE法和氯化苄法提取彩色甜椒基因组DNA。结果表明,CTAB法所提取DNA的质量和纯度较高,以此DNA为模板进行RAPD-PCR分析,DNA扩增效果较好,带形清晰、整齐,说明CTAB法提取的DNA较为完整,能用作RAPD-PCR模板来开展彩色甜椒分子水平的研究。Benzyl chloride method, CTAB, SDS, and ROSE methods were used to extract DNA from color sweet pepper leaves of different fruit color. The results of UV-sepectrophotometry, agarose gel elec- trophoresis and PCR showed that, CTAB method was suitable for color sweet pepper. RAPD-PCR with DNA extracted by CTAB as templates showed that its amplification was good, clear and uniform. DNA extracted by CTAB could be used as templates for molecular biological research in color sweet pepper.

关 键 词：彩色甜椒 DNA提取 RAPD—PCR 

分 类 号：S641[农业科学—蔬菜学] Q78[农业科学—园艺学]