EB病毒BZLF1N基因的克隆与序列分析  

Molecular cloning and sequence analysis of Epstein-Barr virus BZLF1N gene

作　　者：伊强[1] 陈丹[1] 李雯[1] 刘奇才[1] 欧启水[1] 

机构地区：[1]福建医科大学附属第一医院检验科,福州市350005

出　　处：《实用医学杂志》2010年第6期910-912,共3页The Journal of Practical Medicine

基　　金：福建省教育厅高等学校新世纪优秀人才支持计划项目(编号:NCETFJ-0607);福建省卫生厅青年科研基金(编号:2008-1-35)

摘　　要：目的:克隆EB病毒BZLF1N基因编码区的cDNA并对其序列进行分析。方法:采用RT-PCR方法,从B95-8细胞获得BZLF1N基因的cDNA,克隆至pGEM-TEasy载体,选择阳性克隆并进行序列测定。结果:构建的重组载体中含有EB病毒BZLF1N基因的全长序列,与Genebank公布的序列完全一致。结论:获得EB病毒BZLF1N基因的克隆,为进一步的研究奠定了基础。Objective To clone and analyze the full-length cDNA encoding Epstein-Barr virus （EBV） BZLF1N gene. Methods The eDNA of EBV BZLF1N gene was amplified by RT-PCR using the total RNA extracted from B95-8 cell.The PCR product was cloned into pGEM-T Easy vector and then transferred into E.coli DH5α.The positive recombinant clone was analyzed by restriction endonuclease digestion and DNA sequencing. Results The recombinant plasmid had a complete open reading frame of EBV BZLFIN and shared 100% homology with the sequence of EBV BZLF1N in Genebank. Conclusions The cDNA of Epstein-Barr virus BZLF1N gene is cloned successfully,which will lay the foundation for further research.

关 键 词：基因 EB病毒 克隆 

分 类 号：R346[医药卫生—基础医学] R739.63