淫羊藿总黄酮对异丙肾上腺素致心力衰竭大鼠心肌基质金属蛋白酶-2和基质金属蛋白酶-9的影响  

Effects of total flavonoids of epimedium on matrix metall-oproteinase-2,-9 expression in myocardium of a rat model of congestive heart failure induced by isoproterenol

作　　者：王静 宋耀鸿[2] 李宝石[3] 蔡辉[3] 

机构地区：[1]南京市红十字医院心内科,210002 [2]南京市中医院心内科,210001 [3]南京军区南京总医院中西医结合科,210002

出　　处：《实用医学杂志》2010年第6期949-951,共3页The Journal of Practical Medicine

基　　金：南京市科技局科技发展指导计划(编号:2005指导0523)

摘　　要：目的:研究淫羊藿总黄酮(TFE)对异丙肾上腺素所致充血性心力衰竭大鼠心肌中基质金属蛋白酶-2(MMP-2)和MMP-9表达及活性的影响。方法:取Sprague-Dawley雄性大鼠,采用皮下注射异丙肾上腺素(ISO)方法建立心力衰竭模型。将造模成功的动物随机分为模型组(n=15),美托洛尔组(n=12),TFE小剂量组(n=11),TFE中剂量组(n=10),TFE大剂量组(n=12),另取皮下注射生理盐水的正常大鼠作为正常组(n=10)。观察各组动物血流动力学变化,大鼠心肌MMP-2和MMP-9 mRNA表达水平及MMP-2和MMP-9酶活性的变化。结果:与模型组比较,淫羊藿总黄酮可逆转左心室质量指数和血流动力学参数(P<0.05或0.01),减少大鼠心肌MMP-2和MMP-9mRNA的表达并降低MMP-2和MMP-9的酶活性(P<0.05或0.01)。结论:TFE对心力衰竭大鼠心肌MMP-2和MMP-9mRNA的表达以及MMP-2和MMP-9的酶活性均有抑制作用,有助于改善心力衰竭大鼠的心肌重构。Objective To investigate the effects of total flavonoids of epimedium （TFE） on mRNA expression and activities of metalloproteinase-2（MMP-2）, MMP-9 in myocardium of a rat model of congestive heart failure （CHF） induced by isoproterenol.Methods CHF was induced in male Sprague-Dawley rat model was established using subcutaneous injection of isoproterenol. Rat models of CHF were randomized into model group （n = 15）, metoprolol group （n = 12）, TFE-low-dose group（n = 11）, TFE-medium-dose group（n = 10） and TFE-high-dose group（n = 12）. Rats injected with saline were used as normal cotrols（n = 10）. Left ventricular weight index （LVWI） and haemodynamic parameters of rat hears in each group were detected. MMP-2,MMP-9 mRNA expression in rat myocardium was determined by RT-PCR and real-time PCR, MMP-2, MMP-9 activities were examined by gelatin zymography assay. Results Compared with model group of CHF, TFE could dose-dependently rescure the diversities of LVWI and hemodynamic parameters（P 〈 0.05 or 〈 0.01 ）. In addition, TFE could also significantly down-regulated MMP-2, MMP-9 mRNA expression （P 〈 0.05 or 0.01 ） and suppressed activities of MMP-2 and MMP-9. Conclusion TFE can suppress mRNA expression and activities of MMP-2, MMP-9 in myocardium of a rat model of CHF induced by isoproterenol.This indicates that TFE may has a protective role of preventing myocardial remodeling associated with CHF.

关 键 词：心力衰竭 基质金属蛋白酶-2 基质金属蛋白酶-9 淫羊藿总黄酮 

分 类 号：R285.5[医药卫生—中药学]