p,p'-DDD对人乳腺癌细胞的增殖作用  

Study of the Proliferation Effect of DDD on Mammary Cancer Cell MCF-7

作　　者：董力[1] 张明[1] 汤宁[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所毒理室,北京100021

出　　处：《环境与职业医学》2010年第3期171-172,共2页Journal of Environmental and Occupational Medicine

基　　金：中国疾病预防控制中心环境与健康相关产品安全所青年基金

摘　　要：[目的]观察对,对-二氯二苯基二氯乙烷(p,p'-DDD)对人乳腺癌(MCF-7)细胞增殖的影响。[方法]采用体外培养MCF-7细胞增殖试验检测DDD的类雌激素活性,并用生长曲线对其作用机制进行初步探讨。[结果]DDD染毒剂量在3×10-7mol/L、3×10-6mol/L时,存在刺激MCF-7细胞增殖的类雌激素活性(P<0.05),在3×10-6mol/L剂量时其增殖效应最大。细胞生长曲线方面,DDD组在第3、5、7天各时间段细胞数均明显高于溶剂对照组(P<0.05)。[结论]DDD在3×10-7mol/L、3×10-6mol/L具有刺激MCF-7细胞增殖的类雌激素活性。[ Objective ] To study the proliferation effect of p,p＇-DDD on human mammary cancer cell MCF-7. [ Methods ] We detected estrogenic activity of DDD using the MCF-7 cell proliferation experiment in vitro, and analysed its mechanism by means of growth curve. [ Results ] DDD in the dose of 3 ×10^-7 mol/L and 3 ×10^-6mol/L could stimulate the MCF-7 cell proliferation and produce estrogenic activity, and the most proliferation effect dose was 3 ×10^-6 mol/L. Celt cycle analysis of cell proliferation index indicated that DDD group was significantly higher than the control group at the third, fifth, and seventh day. [ Conclusion ] DDD in the dose of 3 ×10^-7mol/L and 3 ×10^-6mol/L could stimulate the MCF-7 cell multiplication.

关 键 词：雌激素 对  对-二氯二苯基二氯乙烷 细胞增殖试验 

分 类 号：R114[医药卫生—卫生毒理学]