反式激活HIV-1LTR的人疱疹病毒6型(HHV-6)基因片段的初步研究  

IDENTIFICATION AND CHARACTERIZATION OF GENE SEGMENTS IN HUMAN HERPESVIRUS 6(HHV-6)B70 FRAGMENT THAT TRANSACTIVATE THE HUMAN IMMUNODEFICIENCY VIRUS 1 PROMOTER

作　　者：魏莘[1] 王岱[1] 张莉[1] 刘淑红[1] 陈启民[1] 耿运琪[1] 

机构地区：[1]南开大学生命科学学院

出　　处：《病毒学报》1998年第4期370-373,共4页Chinese Journal of Virology

基　　金：国家科委基础研究高技术资助

摘　　要：艾滋病（ＡＩＤＳ）主要是由人免疫缺陷病毒（ＨＩＶ）侵入人体后，破坏人的免疫系统造成的。许多流行病学研究已证明，疱疹病毒与ＨＩＶ的共感染可以导致对ＨＩＶ－１启动子的激活，并加速细胞的病理性反应〔１〕，从而加大个体对ＨＩＶ感染的敏感和加快疾病的进程。人疱...Human herpesvirus type 6(HHV-6)can transactivate the promoter of human immunodeficiency virus(HIV)and accelerate cytopathogenic effects in HIV infected human T cells. A large 22.25kb fragment B70 of HHV-6 showed high transactivity in vitro. In this study, we demonstrated that 3 subfragments of B70 can transactivate the HIV promoter in cotransfected CV-1 cells. All these subfragments can not transactivate HIV-LTR structure containing a deleted or mutated enhancer. These subfragments can work together to increase the HIV-LTR directed Luc gene expression dramatically.

关 键 词：人疱疹病毒6型 HHV-6 HIV-1 共转染 

分 类 号：R373[医药卫生—病原生物学]