蝴蝶兰原球茎和丛生芽增殖条件优化  

Optimization of Multiplication Conditions for Phalaenopsis spp. PLB and Adventitious Buds

作　　者：周全[1] 周翔[1] 唐兴国[1] 宋鹏飞[1] 

机构地区：[1]武汉生物工程学院生物工程系,武汉430415

出　　处：《湖北农业科学》2010年第3期595-598,共4页Hubei Agricultural Sciences

摘　　要：分别以蝴蝶兰(Phalaenop spp)原球茎(Protocorm like body,PLB)和幼芽为外植体,研究了蝴蝶兰原球茎和丛生芽增殖的适宜条件。结果表明,适合于原球茎增殖的培养基为1/3MS+TDZ 0.2 mg/L+NAA 1.0 mg/L+蔗糖20 g/L+琼脂8 g/L+活性炭2 g/L+CM 200 mL/L。适合于丛生芽增殖的培养基为1/3MS+TDZ 2.0 mg/L+NAA 0.1 mg/L+蔗糖20 g/L+琼脂8 g/L+活性炭2 g/L+CM 200 mL/L。TDZ的效果好于6-BA。对丛生芽的切割能显著提高增殖倍数。The appropriate multiplication conditions of the Phalaenopsis spp.PLB（Protocorm like body） and adventitious buds were studied using Phalaenopsis spp.PLB and young shoots as the explants respectively.The results showed that the appropriate medium for the multiplication of PLB was： 1 / 3MS ＋ TDZ 0.2 mg/L＋ NAA 1.0mg/L ＋ sucrose 20 g/L ＋ agar 8 g/L ＋ activated charcoal 2 g/L＋CM 200mL / L.The appropriate medium for the multiplication of adventitious buds was： 1 / 3MS ＋TDZ 2.0mg/L ＋ NAA 0.1 mg/L＋sucrose 20 g/L＋agar 8 g/L＋activated charcoal 2 g / L ＋ CM 200mL / L.TDZ was more effective than 6-BA.The cutting of adventitious bud could obviously increase proliferation.

关 键 词：蝴蝶兰 原球茎 丛生芽 增殖 噻重氮苯基脲 

分 类 号：S682.31[农业科学—观赏园艺] Q943.1[农业科学—园艺学]