基于体外抑菌作用筛选的中药注射剂“炎毒热清”  

Screening the Chinese Medicine Injection "Yandureqing" Based on In Vitro Antibacterial Action

作　　者：陈佳娟[1,2] 李剑勇[2] 杨亚军[2] 李新圃[2] 张继瑜[2] 魏小娟[2] 周旭正[2] 

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所/新兽药工程重点实验室,兰州730050

出　　处：《湖北农业科学》2010年第3期635-637,647,共4页Hubei Agricultural Sciences

基　　金：国家"十一五"科技支撑计划项目(2008BADB4B0202)

摘　　要："炎毒热清"中药注射剂是由连翘、金银花、黄芩等中药混合提取配制而成的复方制剂。为了筛选最佳工艺和配方,采用杯碟法分别对不同提取工艺和配方进行了金黄色葡萄球菌、胸膜肺炎放线杆菌、多杀性巴氏杆菌、大肠杆菌4种细菌的体外抑菌试验。结果表明,"炎毒热清"中药注射剂复方A～复方F对金黄色葡萄球菌、胸膜肺炎放线杆菌、多杀性巴氏杆菌的抑菌作用比复方1～复方6的抑菌作用显著,尤其是复方F的抑菌作用最显著。在复方1～复方6中,复方6的抑菌作用较其他复方显著。全部复方对大肠杆菌效果不明显。通过体外抑菌试验可知,复方F的体外抑菌效果最好,可作为预选方进一步研究。＂Yandureqing＂ was Chinese traditional medicine injection.It was prepared from Weeping forsythia,Honeysuckle flower,Radix scutellariae and other medicines.The experiment was to select the best formula through the test of in vitro antibacterial action.Cup method was used to compare different extract technology and prescription with the antibacterial action on Staphylococcus aureus,Actinobacillus pleuropneumoniae,Pasteurella multocida,Escherichia.coli.The formula A～formula F had more antibacterial effect on S.aureus,A.pleuropneumoniae,P.multocida than the formula 1～formula 6.For-mula F was the most antibacterial in the formula A～formula F.Formula 6 was the most antibacterial in the formula 1～formula 6.All formula had no effect on E.coli.Formula F had the best antibacterial effect and needed to further study.

关 键 词：中药注射剂 体外抑菌试验 最小抑菌浓度 

分 类 号：S853.732[农业科学—临床兽医学] S852.61[农业科学—兽医学]