梭曼和沙林对电鳐电器官乙酰胆碱酯酶抑制作用动力学  

Kinetic Constants for the Inhibition of Marcine timlei Acetylcholinesterase by Sarin and Soman

作　　者：陈久祯[1] 王惠芳[1] 李艳军[1] 刘威 温巨芳 

机构地区：[1]北京药物化学研究所

出　　处：《中国生物化学与分子生物学报》1998年第6期790-792,共3页Chinese Journal of Biochemistry and Molecular Biology

摘　　要：梭曼和沙林是毒性很强的有机磷神经毒剂，它们的共同特点是对胆碱酯酶（ＡＣｈＥ，ＥＣ３．１．１．７）都有很强的抑制作用，但两者又不完全相同．过去人们对梭曼和沙林的毒性、毒理研究得较多，而对其对ＡＣｈＥ抑制作用动力学研究得较少，且相互间差异较大．为了对这一...The kinetic constants for the inhibition of Narcine timlei acetylcholinesterase by Sarin and Soman were studied. The K m value for the interaction of Ach I with AChE was 4 40×10 -5 mol·L -1 . The v 0 was equal to 0.00352 s -1 ( A 412 nm value per second). And the dissociation, phosphorylation and bimolecular reaction constants were determined for inhibition of Narcine timlei acetylcholinesterase by Sarin and Soman in the presence of substrate. They were respectively: K i: 17.24×10 7 and 2 54×10 7 mol -1 ·L·min -1 , K d: 4.28×10 -8 and 4.02×10 -7 mol·L -1 , K 2 : 7.38 and 10.22 min -1 . The results indicated that the affinity and inhibitory power of Soman were higher than of Sarin. This may be one of the factors contributing to the difference in toxicity between them.

关 键 词：梭曼 沙林 乙酰胆碱酯酶 抑制作用 动力学 

分 类 号：R827.12[医药卫生—临床医学]