流行性出血热尸检组织中热休克蛋白70mRNA的定位及分布  

Localization and Distribution of HSP70 mRNA and Hantaan Virus in Autopsy Tissues of the Patients with EHF and in Virus infected Cells

作　　者：叶苓[1] 杨守京[1] 刘彦仿[1] 晏培松[1] 

机构地区：[1]第四军医大学病理学教研室

出　　处：《中国病毒学》1998年第4期327-332,共6页Virologica Sinica

基　　金：国家自然科学基金

摘　　要：应用核酸原位分子杂交技术研究了国内不同地区３０例流行性出血热患者尸检组织中病毒ＲＮＡ及ＨＳＰ７０ｍＲＮＡ细胞内定位，同时观察了汉坦病毒感染的ＶｅｒｏＥ６细胞中ＨＳＰ７０的表达情况。结果表明，热休克蛋白７０ｍＲＮＡ在多数组织中均可检测到，分布与病毒ＲＮＡ一致，并且与组织的病理损害有关；体外实验的结果也表明在出血热病毒感染的细胞中有ＨＳＰ７０的高表达。提示热休克蛋白与汉坦病毒的致病以及流行性出血热的发病机理有关。With in situ hybridization technique the localization and distribution of HSP70 mRNA and Hantaan virus RNA in the tissues of 30 cadavers with epidemic hemorrhagic fever (EHF), which were collected from different EHF epidemic foci in China, were examined. HSP70 mRNA was detected in most of the 30 cases and consistent with the localization and distribution of viral RNA. The distribution of HSP70 mRNA was partly related with the pathogenesis of HFRS. The over expression of HSP70 was also found in the virus infected Vero E 6 cells. It suggested that HSP was associated with the infection of Hantaan virus.

关 键 词：热休克蛋白 汉坦病毒 流行性出血热 HSP70MRNA 

分 类 号：R512.8[医药卫生—内科学]