Disector法测量突触的数密度  

COUNTING OF THE NUMERICAL DENSITY OF SYNAPSES USING THE DISECTOR TECHNIQUE

作　　者：张琳[1] 买鸿宴[1] 南燕[1] 黄浩[2] 沈丽[1] 

机构地区：[1]北京医科大学解剖学系,北京100083 [2]北京大学力学系,北京100871

出　　处：《神经解剖学杂志》1998年第4期411-414,共4页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金

摘　　要：Disector法作为新兴的体视学技术已广泛应用于生物组织中粒子数目的计数，它是利用已知距离的相邻平行切片计数三维空间中任意粒子数目的方法。Disector法作为一种三维探针技术无需基于对待测粒子形状、尺寸和空间分布的任何假设，因此，应用Disector法计数神经组织中突触的数目，尤其是空间结构复杂的突触的数目，具有高效、无偏差等明显的优点。A new stercological technique, the disector technique. has been described to count the number of particles in a given volume of tissue. It is a highly efficient and unbiased design-based technique for counting the number of arbitrary particles in 3dimensional space using adjacent parallel section known distance apart. The disector technique requires no assumption about the shape, the size or the distribution of the particles. The approach is an efficient and unbiased technique to quantify synaptic number within a defined nervous tissue, particularly the synapse has complex spatial configurations.

关 键 词：突触 DISECTOR 形态计量学 数密度 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]