昆明小鼠胎鼠心室肌细胞的原代培养  

The primary culture of ventricular cardiomyocytes from embryonal Kunming mice

作　　者：张玲[1] 郭玉君[1] 段明军[1] 侯月梅[1] 

机构地区：[1]新疆医科大学第一附属医院心脏中心,心律失常VIP研究室,新疆乌鲁木齐830011

出　　处：《新疆医科大学学报》2010年第3期255-257,共3页Journal of Xinjiang Medical University

基　　金：国家自然科学基金项目(30760079)

摘　　要：目的探讨胚胎小鼠心室肌细胞分离培养的方法及细胞的存活状况。方法采用胰蛋白酶消化心室肌细胞,差速贴壁2h纯化心肌细胞培养,台盼蓝染色判定心肌细胞存活率。结果心室组织4～6次基本消化完全,48h观察细胞存活率大于90%,生物倒置显微镜下观察,24h时有少部分细胞搏动,48h时细胞交织成网,搏动呈同步性,搏动频率30～150次/min。结论用胰蛋白酶消化法可以成功地分离小鼠胎鼠心室肌细胞,可获得形态、活力良好的心室肌细胞。Objective To establish a method to isolate and culture ventricular cardiomyocytes from embryonal Kunming mice,and evaluate the viability of cultured cardiomyocytes.Methods The ventricular myocardium from fetal mice were digested in trypsin,and the purified cardiocytes were obtained by differential adhesion about 2 hours.The survival rate was assessed by trypan staining.Results The ventricular tissues were almost completely digested through 4-6 times.We found that the cells connected to each other and beat after 24 hours.Trypan staining showed that the survival rate of the cardiocytes cultured was more than 95% on 48 hours.Under the biological invert microscope,the cells connected to each other and some cells began to beat after 24 hours.The cells monolayer formed a network and beated spontaneously at the 30-90 times/min.Conclusion The ventricular cardiomyocytes can be obtained with well morphology and spontaneous beating with trypsin.

关 键 词：胎鼠 心室肌细胞分离 细胞培养 

分 类 号：R318.5[医药卫生—生物医学工程]