小剂量肝素对脓毒症大鼠的早期干预作用  

Effects of low-dose heparin on sepsis rats in early stage

作　　者：逯峰[1] 吕长俊[1] 张晓荣[1] 王晓芝[1] 刘晓立[1] 高原[1] 

机构地区：[1]滨州医学院附属医院,山东滨州256600

出　　处：《山东医药》2010年第13期19-21,共3页Shandong Medical Journal

基　　金：山东省自然科学基金资助项目(Z2007C10)

摘　　要：目的观察小剂量肝素对脓毒症大鼠的早期干预作用。方法雌性Wistar大鼠96只,随机分为假手术组(8只)、脓毒症组(44只)、肝素干预组(44只),以盲肠结扎穿孔法复制大鼠脓毒症模型。于不同时间点观察各组大鼠凝血酶原时间、活化部分凝血酶时间(APTT)及血小板(PLT)、巨噬细胞移动抑制因子(MIF)水平变化;光镜下观察大鼠肺脏组织病理学的改变,计算大鼠48 h病死率。结果肝素干预组MIF水平较脓毒症组降低(P<0.01),肺组织损伤减轻,APTT升高及PLT降低的程度较脓毒症组减轻(P均<0.05)。结论早期应用小剂量肝素可改善脓毒症凝血功能紊乱,明显降低血清MIF水平,减轻肺脏组织损伤程度。Objective To observe the effects of low-dose heparin on sepsis rats in early stage. Methods A total of 96 female Wistar rats was randomly divided into sham operation group（ n = 8 ）, sepsis group（ n = 44） and heparin interfere group （n =44）. Sepsis rats models were prepared by cecal ligation and puncture （CLP）. PT, APTr in plasma and PLT in blood were observed at different times, MIF level was detected by ELISA. The pathological changes in lung tissues were observed with microscope after HE staining, the mortality for 48 hours after CLP was evaluated. Results In heparin interfere group, the MIF level was markedly decreased, and lung injury was lessened（ P 〈 0.01 ） , the changes of APTT and PLT levels were attenuate（ all P 〈 0.05）. Conclusion Low-dose heparin used early could ameliorate the disorder of blood coagulation, and markedly decrease the plasmic MIF level as well as lung injury.

关 键 词：脓毒症 巨噬细胞移动抑制因子 肝素 

分 类 号：R631[医药卫生—外科学]