RD105缺失基因检测法用于结核分枝杆菌新疆“北京家族”临床分离株的鉴定  

Identification of the "Beijing family" strains of clinically isolated Mycobacterium tuberculosis in Xinjiang using an RD105 deletion test

作　　者：吴长东[1] 米利古[1] 龚天美 杨海洲 王远志[1] 李永祥[1] 张向晖[1] 吴万贵[1] 邢建新[1] 袁俐[1] 

机构地区：[1]新疆石河子大学医学院,新疆石河子832002 [2]新疆喀什农三师医院 [3]新疆和田农十四师疾病预防控制中心

出　　处：《中国病原生物学杂志》2010年第3期161-163,167,共4页Journal of Pathogen Biology

基　　金：国家自然科学基金资助项目(No.30960356)

摘　　要：目的通过RD105缺失基因检测法鉴定结核分枝杆菌"北京家族"菌株,了解"北京家族"菌株在新疆地区的分布特征。方法收集新疆结核分枝杆菌临床分离株,采用RD105缺失基因检测法鉴定"北京家族"菌株。统计学分析采用χ2检验。结果共收集到229株结核分枝杆菌临床分离株,采用RD105缺失基因检测法鉴定出"北京家族"菌株100株,占全部菌株的43.7%,非"北京家族"菌株129株,占全部菌株的56.3%,"北京家族"菌株在维吾尔族和汉族病例中所占的比例分别为39.5%(79/200)和72.4%(21/29)。统计学分析"北京家族"菌株在新疆地区汉族和维吾尔族病例中的分布差异有统计学意义(χ2=11.154,P=0.001)。结论结核分枝杆菌"北京家族"菌株在新疆维吾尔族人群中呈一般流行趋势,而在汉族人群中为主要流行菌株。"北京家族"菌株是否为新疆地区的主要流行菌株还有待进一步研究。Objective An RD105 deletion test was used to identify the ＂Beijing family＂ strains of Mycobacterium tuberculosis in order to determine the distribution of ＂Beijing family＂ strains in Xinjiang.Methods M.tuberculosis clinical isolates were collected and identified with an RD105 deletion test.Statistical analysis was performed using the χ2 test.Results In total,229 clinical isolates of M.tuberculosis were collected;these isolates consisted of 100 strains in the ＂Beijing family＂ of M.tuberculosis（43.7%） and 129 strains not in that family（56.3%）.＂Beijing family＂ strains accounted for 39.5% of isolates（79/200） from the Uygur ethnic group and 72.4%（21/29） from the Han ethnic group in Xinjiang.There was a significant difference in the distribution of ＂Beijing family＂ strains of M.tuberculosis in the Uygur and Han ethnic groups in Xinjiang（χ2=11.154,P=0.001）.Conclusion ＂Beijing family＂ strains of M.tuberculosis were typically prevalent in the Uygur ethnic group in Xinjiang but predominant in the Han ethnic group.Whether of not the ＂Beijing family＂ strains of M.tuberculosis are the predominant strains in the region should be studied further.

关 键 词：分枝杆菌 结核 RD105 北京家族基因型 

分 类 号：R378.911[医药卫生—病原生物学]