黄芩素对内毒素诱导的内皮细胞细胞间黏附分子-1表达的影响  

Effect of Baicalein on Expression of Intercellular Adhesion Molecule-1 in Endothelial Cells Stimulated by Lipopolysaccharide

作　　者：李岩[1] 王宏敏[1] 李明[2] 高智群[1] 邝枣园[1] 

机构地区：[1]广州中医药大学基础医学院 [2]广东药学院基础学院

出　　处：《中西医结合心脑血管病杂志》2010年第4期436-438,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

摘　　要：目的研究黄连解毒汤有效成分黄芩素对细菌脂多糖(LPS)诱导的人血管内皮细胞细胞间黏附分子-1(ICAM-1)和抑制蛋白κB(I-κB)表达的影响。方法分别用LPS(终浓度1μg/mL)或LPS+黄芩素(终浓度1μmol/L、10μmol/L、50μmol/L)处理生长良好的ECV-304细胞,用RT-PCR和WesternBlot法检测ICAM-1表达情况和I-κB含量变化。结果LPS刺激ECV-304细胞可促进I-κB降解,上调ICAM-1表达水平,与空白对照组比较有统计学意义(P<0.01);黄芩素预处理能抑制LPS诱导的I-κB降解,降低ICAM-1表达水平,与LPS组比较有统计学意义(P<0.05)。结论黄芩素可通过抑制I-κB降解,影响NF-κB炎症信号途径,下调ICAM-1的表达,这可能是黄连解毒汤抗动脉粥样硬化的作用机制之一。Objective To investigate the effects of baicalein,an effective components of Huanglian Jiedu decoction,on expression of intercellular adhesion molecule - 1 （ICAM - 1） and inhibitor of kB （I - kB） in endothelial cells ECV - 304 induced by lipopolysaccha- ride（LPS）. Methods ECV- 304 cells were cultured in vitro,and treated by different concentration of baicalein（1 μmol/L, 10 μmol/L, 50 μmol/L） with or without LPS（1 μmol/L）. The mRNA and protein expression ＂of ICAM- 1 were determined by reverse tran- scription polymerase chain reaction（RT - PCR）and Western blot, respectively. The expression of I- kB was detected by Western blot. Results LPS up - regulated the expression of ICAM - 1 in endothelial cells significantly, and markedly inhibited the expression of I - kB com- pared with control group（P〈0.01）. Following pretreatment with baicalein, LPS - induced ICAM - 1 expression was down - regulated in both mRNA and protein levels,and degradation of I - kB was inhibited significantly compared with LPS - treatment group（P〈20. 05）. Conclusion Baicalein might effectively down regulated ICAM - 1 expression in LPS - induced endothelial cells through inhibiting the degradation of I - kβ. It might be associated with the amelioration of atherosclerosis damage by Huanglian jiedu decoction.

关 键 词：黄芩素 血管内皮细胞 内皮细胞细胞间黏附分子-1 抑制蛋白κB 内毒素 

分 类 号：R285.5[医药卫生—中药学]