脱氮副球菌W12在游离生长与附着生长条件下对吡啶去除作用的研究  

Removal of Pyridine Using Suspended and Attached Growing Bacterium of Paracoccus denitrificans W12

作　　者：俞蓉[1] 赵翠[1] 刘江江[2] 陈吕军[2] 温东辉[1] 

机构地区：[1]北京大学环境科学与工程学院,北京100871 [2]清华大学环境科学与工程系,北京100084

出　　处：《环境科学》2010年第4期1053-1058,共6页Environmental Science

基　　金：国家高技术研究发展计划(863)项目(2006AA06Z336)

摘　　要：分离到1株吡啶降解菌——脱氮副球菌(Paracoccus denitrificans W12),将其附着生长于颗粒活性竹炭(ABC)制备成生物活性竹炭(BABC),分别以游离菌和固着菌开展对水中吡啶的去除实验.结果表明,0.31g·L-1游离态W12菌可在26.5～48.9h内将48.70～1399mg·L-1的吡啶完全降解;附着于ABC的W12菌不仅保持其降解能力,而且BABC通过吸附与降解的协同作用更高效地去除吡啶,当温度为35℃,BABC投加量为10.0g·L-1时,692.2mg·L-1的吡啶在前3.6h主要通过吸附作用降低52%,其后主要通过生物降解作用至23.7h被基本去除,随着BABC投加量和处理批次的增加,吡啶去除效率显著提高.研究进一步从微观结构上对BABC去除水中吡啶的协同作用机制进行了探讨.In this study,a pyridine-degrading bacterium,Paracoccus denitrifican W12,was isolated. It was cultivated to grow on the surface of activated bamboo charcoal （ ABC） particles so that the ABC turned into biological activated bamboo charcoal （ BABC） covered with biofilm of the W12. Free cells of the W12 and the BABC were separately tested in removing pyridine from aqueous solution. The results showed that 0. 31 g·L^-1 suspended growing-W12 completely degraded 48. 70-1 399 mg·L^-1 of pyridine within 26. 5-48. 9 h,while the BABC （ attached growing-W12 ） degraded pyridine much more efficiently due to the combination of biodegradation and adsorption. When the dosage of BABC was 10. 0 g·L^-1 at the temperature of 35℃ ,692. 2 mg·L^-1 of pyridine was decreased by 52% in the first 3. 6 h mainly by adsorption,then was totally removed within 23. 7 h mainly by biodegradation. Increasing the dosage of BABC or batch of treatment promoted the efficiency of pyridine removal remarkably. The synergistic mechanism of BABC removing pyridine from aqueous solution was further discussed on the basis of its microstructure.

关 键 词：吡啶降解菌 生物活性竹炭(BABC) 吸附 生物降解 

分 类 号：X703.1[环境科学与工程—环境工程] X172