一种新发生的大豆茎枯病病原菌鉴定  

Pathogen identification of a newly occurred soybean stem blight in China

作　　者：崔友林[1] 段灿星[1] 丁俊杰[2] 王晓鸣[1] 武小菲[1] 朱振东[1] 

机构地区：[1]中国农业科学院作物科学研究所,国家农作物资源与基因改良重大工程,北京100081 [2]黑龙江省农业科学院佳木斯分院,黑龙江佳木斯154007

出　　处：《中国油料作物学报》2010年第1期99-103,共5页Chinese Journal of Oil Crop Sciences

基　　金："十一五"国家科技支撑计划(2006BAD08A15);中国农业科学院作物科学研究所中央级公益性科研院所基本科研业务费专项(082060302-06)

摘　　要：鉴定了一种新发生的大豆茎枯病病原菌。在酸化PDA培养基上所有病原菌分离物菌落呈白色,气生菌丝呈密集的卷毛状,有时部分区域显黄绿色;培养基背面开始无色,随后产生大的、扩展的黑色子座。在接种的大豆茎秆上产生大小不等的黑色子座和分散的分生孢子器。共产生2类分生孢子,其中α型分生孢子丰富,无色,单孢,椭圆至纺锤状形,大小4.05～7.57μm×1.48～3.25μm,含2个油滴;β型分生孢子极少见,无色,线形。在培养基及大豆茎秆上未发现有性态。致病性测定表明选择的分离物引起合丰25大豆100%植株发病和种子腐烂。用AluI、RsaI和HhaI酶切通用引物对ITS4/ITS5扩增的rDNA-ITS产物,所有分离物都产生与大豆拟茎点种腐病菌Phomopsis longicolla一致的酶切谱带;序列比对发现测序的2个分离物ITS序列与GenBank中9个P.longicolla分离物ITS序列100%同源。这是我国首次报道大豆田间发现P.longicolla,而且能引发严重的大豆茎枯病。The pathogen causing soybean stem blight disease was newly identified.The isolate produced floccose dense white colonies with occasional green-yellowish areas on acidified PDA medium was newly identified.The stromata were large,black and widespread.It was observed that the pycnidia produced both alpha-and beta-conidia when inoculated on soybean stem.Alpha-conidia were hyaline,ellipsoidal to fusiform,and guttulate with sizes of 4.05 to 7.57μm ×1.48to 3.25μm.Beta-conidia were rare,hyaline,filiform and hamate.Four isolates selected caused seeds rotten and 12-day-old seedlings diseased when inoculated on soybean cultivar Hefeng 25.To indentified the pathogen,rDNA-ITS fragment was amplified using universal primer ITS4 and ITS5,and followed by digesting with Alu I,Rsa I and Hha I restriction enzymes. Phomopsis longicolla characteristic band spectrum was shown in every isolate PCR products.Two isolated ITS sequences were identical to 9 isolates of P.longicolla in GenBank.The results indicated that the pathogen of severe soybean stem blight was just P.longicolla.It was the first report of P.longicolla being isolated from soybean in China.

关 键 词：大豆 大豆拟茎点种腐病菌 大豆茎枯病 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]