基于元分析的大豆胞囊线虫抗性QTL的整合  

Integration of QTLs related to soybean cyst nematode resistance based on meta-analysis

作　　者：张闻博[1,2] 蒋洪蔚[2] 李灿东 邱鹏程[1,2] 齐照明[1,2] 刘春燕[2] 姜威[1,2] 王晶[1,2] 胡国华[2,4] 陈庆山[1] 

机构地区：[1]东北农业大学农学院,哈尔滨150030 [2]黑龙江省农垦科研育种中心,哈尔滨150090 [3]黑龙江省农科院佳木斯分院,佳木斯154007 [4]国家大豆工程技术研究中心,哈尔滨150050

出　　处：《中国油料作物学报》2010年第1期104-109,共6页Chinese Journal of Oil Crop Sciences

基　　金：引进国际先进农业科学技术计划(948计划)项目(2006-G1(A));国家高技术研究发展计划(863计划)项目(2006AA100104-3);黑龙江省博士后科研启动基金(LHK-04014);国家重大专项(2009ZX08009-D13B)

摘　　要：以2004年发布的大豆公共遗传图谱soymap2为参考图谱,共搜集整理了自1994年以来已报道的与抗大豆胞囊线虫相关的151个QTLs,通过BioMercator2.1和公共标记将相关QTLs映射整合到大豆公共遗传连锁图谱soymap2上,并利用元分析技术发掘"真实"QTL。本文共发掘出与抗大豆胞囊线虫1、2、3、4、5和14号生理小种相关的16个"真实"QTL,其中有四个位点的图距小于1.5cM,一个位点的图距小于5cM,分布于A2、E和G连锁群,其中G连锁群上5.11cM处的定位区间包含已报道的Rhg1位点;发现在G连锁群上有一个定位区间控制1,4号生理小种,在B1连锁群上有一个定位区间控制2,5号生理小种,可见这些位点具有兼抗性。According to soybean public map soymap2 （published in 2004）,151 QTLs related to soybean cyst nematode resistance were collected from markers reported since 1994. They were integrated and projected to the reference soymap2 using BioMercator2.1 software. 16 ＂real＂ QTLs and their corresponding markers were obtained using meta-analysis method to narrow down the confidence interval. The ＂real＂ QTLs included markers related cyst nematode resistance to race 1,2,3,4,5,and 14. Part of them distributed on A2,E,and G linkage groups （LGs）. Four of them had intervals less than 1.5 cM and one of them less than 5 cM. Rhg1,a previously reported locus,was found on LG G with 5.11 cM. Two of them located on LG B1 which related to race 2 and 5,another two on LG G related to race1 and 4. These results indicated double resistances to soybean cyst nematode and offered a basis for gene mining or molecular breeding in soybean.

关 键 词：大豆胞囊线虫 QTL的映射 元分析 真实QTL 

分 类 号：Q348[生物学—遗传学] S565.103[农业科学—作物学]