甘蓝型油菜小孢子离体培养条件改良  

Modification of microspore in vitro culture of Brassica napus L.

作　　者：吴士红[1] 戚存扣[2] 

机构地区：[1]南京农业大学农学院,江苏南京210095 [2]江苏省农业科学院经济作物研究所,江苏南京210014

出　　处：《中国油料作物学报》2010年第1期124-127,共4页Chinese Journal of Oil Crop Sciences

基　　金：江苏省自主创新项目(CX07705);国家973计划项目(2006CB101605);农业部948项目(2006-G04)

摘　　要：应用低含油量甘蓝型油菜品系1064与高含油量品系H105杂种F1花蕾进行小孢子培养,研究了两个种植地点的油菜小孢子产胚率的影响。对长度在0.4cm～0.6cm,0.6cm～0.8cm以及0.8cm以上的胚分别培养,并比较了秋水仙碱悬浮处理离体小孢子、浸根和滴芽3种染色体加倍方法的加倍效率。结果表明,种植在西宁的材料小孢子产胚率比南京高,为8.6枚/皿;0.6cm～0.8cm大的胚转接到B5固体培养基,其成苗率高达48.53%;3种加倍处理方法中以50mg/L秋水仙碱悬浮小孢子48h染色体加倍效率最高,达到46.23%。本研究构建了含有123个株系的DH群体,可用于油菜含油量性状的QTL分析。Brassica napus L. microspores from F1 derived from low oil content line 1064 and high oil content line H105 hybrid were cultured for establishing double haploid （DH） population. F1 were planted in field in Nanjing and Xining,respectively. Embryogenesis rate difference was observed between two locations. Regeneration rate was recorded using embryos with sizes of 0.4cm to 0.6cm,0.6cm to 0.8cm and 0.8cm. Doubling efficiency of three colchicine treatment methods were also investigated. Results showed that embryogenesis rate was higher and reached up to 8.6 embryos per plate in Xining than that in Nanjing. 0.6cm to 0.8cm sized embryos transferred to B5 solid medium gave a higher regeneration ratio of 48.53%.High doubling efficiency of 46.23% was obtained from 50 mg/L colchicine suspension treatment of microspores for 48h.A DH population of 123 lines was obtained in this study. It can be used as the basic population for QTL analysis of oil content in B.napus L.

关 键 词：甘蓝型油菜 小孢子培养 产胚率 

分 类 号：S565.403[农业科学—作物学]