花生青枯菌红安分离物的鉴定  

Identification and mutation of Ralstonia solacearum Hongan isolate

作　　者：晏立英[1] 黄家权[1] 雷永[1] 王圣玉[1] 廖伯寿[1] 

机构地区：[1]中国农业科学院油料作物研究所农业部油料作物生物学重点开放实验室,湖北武汉430062

出　　处：《中国油料作物学报》2010年第1期144-146,共3页Chinese Journal of Oil Crop Sciences

基　　金：863计划项目(2006AA10A115)

摘　　要：从湖北红安发病花生根部分离到一个细菌分离物2C1,对该分离物进行了TZC显色反应,致病力的测定,16S rRNA、23S rRNA和鞭毛蛋白基因filC的PCR扩增,16S rRNA PCR扩增产物的序列分析。结果显示,分离物2C1在TZC培养基上表现出青枯菌的典型菌落形态,对花生具有强致病性,PCR扩增获得与预期大小的产物片段;16S rRNA核苷酸序列具有与其它青枯菌分离物99%左右的一致性。上述结果表明:2C1是具有强致病力的花生青枯菌分离物。A bacterium isolate 2C1 from the root of diseased peanut seedling from Hubei Hongan was identified by its pathogenecity to peanut,PCR amplification of 16S rRNA,23S rRNA and filC gene and 16S rRNA PCR product sequencing.The results revealed that 2C1 was virulent to peanut and showed the typical bacterial wilt symptom,and it amplified the prospected size of fragments of 16S rRNA,23S rRNA and filC gene of Ralstonia solanacearum by PCR,and its 16S rRNA PCR product showed 99% nucleotide matching with those strains of R.solanacearum.

关 键 词：花生 青枯菌 致病性 鉴定 

分 类 号：S435.652[农业科学—农业昆虫与害虫防治]